Proteomics in plasma of ovariectomized rats and those exposed to estradiol valerate

Publication date: Available online 18 October 2017 Source:The Journal of Steroid Biochemistry and Molecular Biology Author(s): Hai Jiang, Wenpei Bai, Wenjuan Wang, Ke Wang, Jing Jia, Jing Zhang, He Diao, Lihua Qin The menopausal period, an inevitable physiological process for women, is frequently associated with physiological and psychological dysfunction attributable to substantial fluctuation and gradual decrease in female hormones induced by ovarian failure, leading to corresponding symptoms and diseases that impact multiple systems in the body to varying degrees. As prior studies have focused primarily on menopausal syndrome-related pathophysiological changes and hormone replacement therapy effects, here we approached menopausal disease incidence risk and pathogenesis through systemic plasma proteomics analysis. Female Sprague-Dawley rats were randomly divided into sham, ovariectomized, and estrogen treatment after ovariectomy groups (n=9 per group). Tandem Mass Tag quantitative proteomics analysis of their plasma identified over 900 proteins by MS. Between group fold change of >1.2 and p<0.05 (Student’s t test) identified 121 (including 36 up-regulated and 85 down-regulated), 117 (69 up-regulated and 48 down-regulated), and 109 (41 up-regulated and 68 down-regulated) differentially expressed proteins between groups, respectively. Of these, 5 (GHR, LIFR, apoA IV, RTN, and Lin28b) were verified by parallel reaction monitoring to be reliable. Furth...
Source: The Journal of Steroid Biochemistry and Molecular Biology - Category: Biochemistry Source Type: research