Normal and Cancerous Tissues Release Extrachromosomal Circular DNA (eccDNA) into the Circulation

Cell-free circulating linear DNA is being explored for noninvasive diagnosis and management of tumors and fetuses, the so-called liquid biopsy. Previously, we observed the presence of small extrachromosomal circular DNA (eccDNA), called microDNA, in the nuclei of mammalian tissues and cell lines. Now, we demonstrate that cell-free microDNA derived from uniquely mapping regions of the genome is detectable in plasma and serum from both mice and humans and that they are significantly longer (30%–60%>250 bases) than cell-free circulating linear DNA (~150 bases). Tumor-derived human microDNA is detected in the mouse circulation in a mouse xenograft model of human ovarian cancer. Comparing the microDNA from paired tumor and normal lung tissue specimens reveals that the tumors contain longer microDNA. Consistent with human cancers releasing microDNA into the circulation, serum and plasma samples (12 lung and 11 ovarian cancer) collected prior to surgery are enriched for longer cell-free microDNA compared with samples from the same patient obtained several weeks after surgical resection of the tumor. Thus, circular DNA in the circulation is a previously unexplored pool of nucleic acids that could complement miRNAs and linear DNA for diagnosis and for intercellular communication. Implications: eccDNA derived from chromosomal genomic sequence, first discovered in the nuclei of cells, are detected in the circulation, are longer than linear cell-free DNA, and are released from n...
Source: Molecular Cancer Research - Category: Cancer & Oncology Authors: Tags: Genomics Source Type: research

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Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
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Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research
Condition:   Epithelial Ovarian, Fallopian Tube, or Primary Peritoneal Cancer Interventions:   Drug: TQB2450;   Drug: Anlotinib Sponsor:   Chia Tai Tianqing Pharmaceutical Group Co., Ltd. Not yet recruiting
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Source: Journal of Geriatric Oncology - Category: Cancer & Oncology Source Type: research
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Source: Fight Aging! - Category: Research Authors: Tags: Daily News Source Type: blogs
Source: Gynecological Endocrinology - Category: Endocrinology Authors: Source Type: research
Source: Cancer Management and Research - Category: Cancer & Oncology Tags: Cancer Management and Research Source Type: research
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Source: Future Medicine: Pharmacogenomics - Category: Genetics & Stem Cells Authors: Source Type: research
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Source: Journal of Community Genetics - Category: Genetics & Stem Cells Source Type: research
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Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
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