Molecular Effects of Stromal-Selective Targeting by uPAR-Retargeted Oncolytic Virus in Breast Cancer

The tumor microenvironment (TME) is a relevant target for novel biological therapies. MV-m-uPA and MV-h-uPA are fully retargeted, species-specific, oncolytic measles viruses (MV) directed against murine or human urokinase receptor (PLAUR/uPAR), expressed in tumor and stromal cells. The effects of stromal-selective targeting by uPAR-retargeted MVs were investigated. In vitro infection, virus-induced GFP expression, and cytotoxicity by MV-h-uPA and MV-m-uPA were demonstrated in human and murine cancer cells and cancer-associated fibroblasts in a species-specific manner. In a murine fibroblast/human breast cancer 3D coculture model, selective fibroblast targeting by MV-m-uPA inhibited breast cancer cell growth. Systemic administration of murine-specific MV-m-uPA in mice bearing human MDA-MB-231 xenografts was associated with a significant delay in tumor progression and improved survival compared with controls. Experiments comparing tumor (MV-h-uPA) versus stromal (MV-m-uPA) versus combined virus targeting showed that tumor and stromal targeting was associated with improved tumor control over the other groups. Correlative studies confirmed in vivo viral targeting of tumor stroma by MV-m-uPA, increased apoptosis, and virus-induced differential regulation of murine stromal genes associated with inflammatory, angiogenesis, and survival pathways, as well as indirect regulation of human cancer pathways, indicating viral-induced modulation of tumor–stroma interactions. These data...
Source: Molecular Cancer Research - Category: Cancer & Oncology Authors: Tags: Oncogenes and Tumor Suppressors Source Type: research

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Conclusion: MeV vaccine is useful and safe as anticancer therapy with a notable impact on the local Iraqi breast cancer AMJ13 cells.
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research
Poshmaal Dhar1 and Julie McAuley2* 1Faculty of Health, School of Medicine, Deakin University, Geelong, VIC, Australia 2Department of Microbiology and Immunology, Peter Doherty Institute, University of Melbourne, Melbourne, VIC, Australia The family of cell surface (cs-) mucins are constitutively expressed at the cell surface by nearly all epithelial cells, beneath the gel-mucin layer. All cs-mucin family members have structural features that enable them to act as a releasable decoy barrier to mucosal pathogens, by providing ligands for pathogen binding and the ability to shed the bound extracellular domain. Due ...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Conclusion: The incidence rate of non-communicable diseases increased over the decade and was associated with increased mortality and disability, reduced quality of life, and increased health-care costs, indicating an urgent need to establish prevention and control programs. The rising trend in the incidence of cancers may also become a health care issue in Saudi Arabia in the coming years. PMID: 30834424 [PubMed - in process]
Source: Saudi Medical Journal - Category: Middle East Health Tags: Saudi Med J Source Type: research
In this study, we show that calorie restriction is protective against age-related increases in senescence and microglia activation and pro-inflammatory cytokine expression in an animal model of aging. Further, these protective effects mitigated age-related decline in neuroblast and neuronal production, and enhanced olfactory memory performance, a behavioral index of neurogenesis in the SVZ. Our results support the concept that calorie restriction might be an effective anti-aging intervention in the context of healthy brain aging. Greater Modest Activity in Late Life Correlates with Lower Incidence of Dementia ...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Geetanjali Lal, Maitreyi S. Rajala
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Beloved Hollywood celebrities, famous politicians or members of the British royal family: no better advertisement for fitness tracker producers and health tech companies. As models, actors and actresses are highly influential people, their early adoption of digital solutions could also push the masses towards living more healthily with technologies. On the other hand, celebrities are inclined to follow questionable health trends, too, which go against decades of medical evidence. Those examples, everyone should rather reject. Wearables conquered Hollywood, the White House, and the British royal family Celebrities are all a...
Source: The Medical Futurist - Category: Information Technology Authors: Tags: Social media in Healthcare celebrities digital health digital technologies digital technology famous fitness future health influencer health influencers Hollywood trackers trends wearables wellness Source Type: blogs
As a pharmacist, Kathy James considers herself well educated about the importance of getting regular cancer screenings. Even though the 55-year-old had no history of cancer in her family, she never skipped her regular mammograms, and she gave herself regular breast exams. So she was dumbfounded when, during one of those self-exams in May 2017, she felt a marble-size lump in her left breast. A visit to the doctor confirmed it. “The radiologist came in with his hands in his pockets and looked down and said, ‘It doesn’t look good,'” James says. After a biopsy, James and her husband learned she had meta...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized breast cancer news Frontiers of Medicine Source Type: news
Publication date: December 2017Source: Food Science and Human Wellness, Volume 6, Issue 4Author(s): Deepu Mathew, Lidiya John P., Manila T.M., Divyasree P., Sandhya Rajan V.T.K.AbstractMolecular mechanism behind the therapeutic potential of pigeon pea over the human diseases such as rheumatoid arthritis, breast cancer, type II diabetes, malaria, measles and sickle cell disease were revealed through docking of GC–MS identified phyto-compound ligands with candidate disease proteins. Of the 242 ligands, three dimensional structures of 47 compounds had to be drawn using ChemSketch and the remaining structures were retrie...
Source: Food Science and Human Wellness - Category: Food Science Source Type: research
People who don’t get vaccinated are the most likely reason for the steady increase in the rate of measles and major outbreaks in the United States, according to an analysis released Tuesday. The findings, published in JAMA Pediatrics, add to the body of evidence linking failure to vaccinate with the spread of the highly infectious […]Related:Breast-cancer death rate drops almost 40 percent, saving 322,000 lives, study saysNobel in physiology, medicine awarded to three Americans for discovery of ‘clock genes’A model decided to tattoo her eyeball. She’s now partially blind and in ‘excruciating’ pain.
Source: Washington Post: To Your Health - Category: Consumer Health News Source Type: news
Breast cancer death rates declined almost 40 percent between 1989 and 2015, averting 322,600 deaths, the American Cancer Society reported Tuesday. Breast cancer death rates increased by 0.4 percent per year from 1975 to 1989, according to the study. After that, mortality rates decreased rapidly, for a 39 percent drop overall through 2015.  The report, the […]Related:Failure to vaccinate is likely driver of U.S. measles outbreaks, report saysNobel in physiology, medicine awarded to three Americans for discovery of ‘clock genes’A model decided to tattoo her eyeball. She’s now partially blin...
Source: Washington Post: To Your Health - Category: Consumer Health News Source Type: news
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