Spectrophotometric assays for measuring redox biomarkers in blood and tissues: the NADPH network.

Spectrophotometric assays for measuring redox biomarkers in blood and tissues: the NADPH network.
Redox Rep. 2017 Oct 31;:1-10
Authors: Veskoukis AS, Margaritelis NV, Kyparos A, Paschalis V, Nikolaidis MG
Abstract
Nicotinamide adenine dinucleotide (NAD(+)/NADH) along with its phosphorylated form (NADP(+)/NADPH) are two molecules ubiquitously present in all organisms, and they play key roles as cofactors in fundamental catabolic and anabolic processes, respectively. The oxidation of NADPH to NADP(+) initiates a cascade of reactions, where a network of molecules is implicated. The molecules of this cascade form a network with eminent translational potential in redox metabolism. A special point of interest is that spectrophotometric assays have been developed both for NADH/NADPH and the molecules directly regulated by them. Therefore, crucial molecules of the NADPH-dependent redox network can be measured, and the results can be used to assess the bioenergetic and/or oxidative stress status. The main aim of this review is to collectively present the NADPH-related molecules, namely NADPH, NADH, NAD(+) kinase, NADPH oxidase, peroxiredoxin, thioredoxin, thioredoxin reductase, and nitric oxide synthase, that can be measured in blood and tissues with the use of a spectrophotometer, which is probably the most simple, inexpensive and widely used tool in biochemistry. We are providing the researchers with reliable and valid spectrophotometric as...

Source: Redox Report : communications in free radical research - October 31, 2017 Category: Biochemistry Authors: Veskoukis AS, Margaritelis NV, Kyparos A, Paschalis V, Nikolaidis MG Tags: Redox Rep Source Type: research