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Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news

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In conclusion, this study shown excellent compatibility of A. oryzae DIA-MF using aguamiel as an economical substrate for the FOS production under a simple bioprocess.
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Authors: Machowicz-Matejko E, Furmańczyk A, Zalewska ED Abstract The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar (PDA) and maltose (MA) (Difco) media using standard laboratory techniques. Cultures were identified using classical and molecular methods. Macro- and microscopic colony morphology was characteristic of fungi from the genus Aspergillus (restricted growth series), most probably Aspergillus penicillioides Speg. Molecular analysis of the following rDNA regions: ITS1, ITS2, 5.8S, 28S rDNA, LSU and ...
Source: Polish Journal of Microbiology - Category: Microbiology Tags: Pol J Microbiol Source Type: research
In this study the localization and antifungal activity of the lung surfactant protein D (SP-D) was studied in an environment mimicking the lung lining. To this end Calu-3 cells were grown on an air-liquid interface allowing them to polarize and to produce mucus at their apical surface. Additionally, neutrophils were added to study their role in fungal clearance. Two fungal pathogens were used for these experiments: Candida albicans and Aspergillus fumigatus, both of clinical relevance. During fungal infection SP-D localized strongly to both fungal surfaces and stayed bound through the different stages of infection. Further...
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research
This study investigated the influence of temperature and water activity (aw) on the growth and biosynthesis of Ochratoxin A (OTA) by Aspergillus carbonarius and Aspergillus ochraceus, and the influence of climate change on the production potential of this mycotoxin in coffee was predicted. Central Composite Rotatable Design with aw ranging from 0.91 to 0.99 and temperatures from 17 to 38 °C was used. A. carbonarius CCDCA 10288 and 10293 showed optimal growth in aw intervals from 0.935 to 0.965 and temperatures between 25 and 32 °C while A. ochraceus CCDCA 10211 and 10212 showed aw intervals from 0.940 to 0.990 ...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
In this study, the Penicillium citrinum nuclease P1 gene (nuc P1) was heterologously expressed in A. niger Bdel4 which barely produces extracellular protein. Multiple copies of nuc P1 was recombinantly expressed in A. niger via 2A peptide, obtaining the maximal enzymatic activity of 77.6 U/mL. After Ni-chelate-affinity purification, the specific activity reached 32.4 U/mg, with the optimum temperature of 65 °C and the optimum pH of 5.3, respectively. The recombinant nuclease P1 was activated by the addition of Mg2+, Zn2+ and Cu2+, and its activity was inhibited by the addition of Ca2+, Fe2+, Mn2+, Ni2+, Co2+, Mg2+, K...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
Publication date: Available online 14 December 2018Source: Process BiochemistryAuthor(s): Jônatas Carvalho Silva, Pedro Renann Lopes de França, Allan Henrique Felix de Melo, Maria Teresa Neves-Petersen, Attilio Converti, Tatiana Souza PortoAbstractProduction of total polygalacturonase (PG) and endo-polygalacturonases (endoPG) from Aspergillus aculeatus URM4953 was optimized in submerged fermentation using passion fruit peel as substrate for pectin hydrolysis in hog plum juice. The highest activities of PG (2.92 ± 0.12 U/mL) and endoPG (6.51 ± 0.04 U/mL) were obtained u...
Source: Process Biochemistry - Category: Biochemistry Source Type: research
Publication date: Available online 13 December 2018Source: Journal of Structural BiologyAuthor(s): Markus Böger, Johan Hekelaar, Sander S. van Leeuwen, Lubbert Dijkhuizen, Alicia Lammerts van BuerenAbstractGalactooligosaccharides (GOS) are prebiotic compounds synthesized from lactose using bacterial enzymes and are known to stimulate growth of beneficial bifidobacteria in the human colon. Bacteroides thetaiotaomicron is a prominent human colon commensal bacterial species that hydrolyzes GOS using an extracellular Glycosyl Hydrolase (GH) family GH53 endo-galactanase enzyme (BTGH53), releasing galactose-based products f...
Source: Journal of Structural Biology - Category: Biology Source Type: research
Publication date: Available online 11 December 2018Source: Food ChemistryAuthor(s): Maryam Khan, Qayyum HusainAbstractβ-Galactosidase is vital to dairy industries because it catalyzes the hydrolysis of lactose into glucose and galactose making it useful for lactose intolerant patients to consume milk and its products. The present study demonstrates the effect of metal ions commonly found in milk, namely Zn2+, K+, Ca2+ and Mn2+ on the activity of native and polyaniline chitosan nanocomposites bound Aspergillus oryzae β-galactosidase. In polyaniline chitosan silver nanocomposite adsorb...
Source: Food Chemistry - Category: Food Science Source Type: research
Date: Thursday, December 13, 2018Year: 2019Location: London, UKContent-type: current_conferences
Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
Date: Thursday, December 13, 2018Year: 2019Location: Holiday Inn Atrium | SingaporeContent-type: current_conferences
Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
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