Analysis of Cotranslational Polyamine Sensing During Decoding of ODC Antizyme mRNA.

Analysis of Cotranslational Polyamine Sensing During Decoding of ODC Antizyme mRNA. Methods Mol Biol. 2018;1694:309-323 Authors: Palanimurugan R, Gödderz D, Kurian L, Jürgen Dohmen R Abstract Polyamines are essential poly-cations with vital functions in all cellular systems. Their levels are controlled by intricate regulatory feedback mechanisms. Abnormally high levels of polyamines have been linked to cancer. A rate-limiting enzyme in the biosynthesis of polyamines in fungi and higher eukaryotes is ornithine-decarboxylase (ODC). Its levels are largely controlled posttranslationally via ubiquitin-independent degradation mediated by ODC antizyme (OAZ). The latter is a critical polyamine sensor in a feedback control mechanism that adjusts cellular polyamine levels. Here, we describe an approach employing quantitative western blot analyses that provides in vivo evidence for cotranslational polyamine-sensing by nascent OAZ in yeast. In addition, we describe an in vitro method to detect polyamine binding by antizyme. PMID: 29080176 [PubMed - in process]
Source: Cancer Control - Category: Cancer & Oncology Authors: Tags: Methods Mol Biol Source Type: research