Erythropoietin inhibits osteoblast function in myelodysplastic syndromes via the canonical Wnt pathway.

Erythropoietin inhibits osteoblast function in myelodysplastic syndromes via the canonical Wnt pathway. Haematologica. 2017 Oct 27;: Authors: Balaian E, Wobus M, Weidner H, Baschant U, Stiehler M, Ehninger G, Bornhäuser M, Hofbauer LC, Rauner M, Platzbecker U Abstract The effects of erythropoietin on osteoblasts and bone formation are controversially discussed. Since patients with myelodysplastic syndromes often display excessively high erythropoietin level, we aimed to analyze the effect of erythropoietin on osteoblast function in myelodysplastic syndromes and define the role of Wnt signaling in this process. Expression of osteoblast-specific genes and subsequent osteoblasts mineralization was increased in mesenchymal stromal cells from healthy young donors by in vitro erythropoietin treatment. However, erythropoietin failed to increase osteoblasts mineralization in old healthy donors and patients with myelodysplasia, whereas the basal differentiation potential of the latter was already significantly reduced compared to age-matched controls (p<0.01). This was accompanied by a significantly reduced expression of genes of the canonical Wnt pathway. Treatment of these cells with erythropoietin further inhibited the canonical Wnt pathway. Exposure of murine cells (C2C12) to erythropoietin also demonstrated a dose-dependent inhibition of TCF/LEF promoter activity (max. at 500 IU/ml, -2.8-fold, p<0.01). The decreased differentiatio...
Source: Haematologica - Category: Hematology Authors: Tags: Haematologica Source Type: research