IrC2/Bf - A yeast and Borrelia responsive component of the complement system from the hard tick Ixodes ricinus.

IrC2/Bf - A yeast and Borrelia responsive component of the complement system from the hard tick Ixodes ricinus. Dev Comp Immunol. 2017 Oct 20;: Authors: Urbanová V, Hajdušek O, Šíma R, Franta Z, Hönig-Mondeková H, Grunclová L, Bartošová-Sojková P, Jalovecká M, Kopáček P Abstract Ticks possess components of a primordial complement system that presumably play a role in the interaction of the tick immune system with tick-borne pathogens and affect their transmission. Here we characterized a novel complement component, tagged as IrC2/Bf, from the hard tick Ixodes ricinus, the principal vector of Lyme disease in Europe. IrC2/Bf is a multi-domain molecule composed of 5-7 CCP modules, varied by alternative splicing, followed by a von Willebrand factor A domain and a C-terminal trypsin-like domain. The primary structure and molecular architecture of IrC2/Bf displays the closest homology to the C3-complement component convertases described in horseshoe crabs. The irc2/bf gene is mainly expressed in the tick fat body associated with the trachea and, as determined by western blotting, the protein is present in low amounts in tick hemolymph. Expression of irc2/bf mRNA was significantly up-regulated in response to the intra-hemocoelic injection of the yeast Candida albicans and all tested Borrelia sp. strains (B. burgdorferi NE5264, B. burgdorferi CB26, B. garinii MSLB, B. afzelii CB43...
Source: Developmental and Comparative Immunology - Category: Allergy & Immunology Authors: Tags: Dev Comp Immunol Source Type: research

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This study was conducted to identify TBPs harbored by water buffaloes and to characterize these pathogens molecularly. One hundred water buffalo blood samples collected from three areas in Bohol, Visayas region, Philippines were screened for various TBPs using pathogen-specific PCR assays. TBPs were detected in 46% of the samples (39% singly infected, 7% coinfected). The pathogens detected were Anaplasma marginale (29%), Babesia bovis (21%), and B. bigemina (3%). None of the blood samples were positive for Theileria annulata, T. orientalis, and B. ovata. A. marginale infection rates were significantly higher (37.5%) among ...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
We report the structural, morphological and optical activities of a paramagnetic manganite (La0.33Ca0.67MnO3) synthesized at 900 °C. A simple method of formation of complex was employed. A complex was formed between a pre-prepared manganite dissolved in HCl and reacted with an organic ligand (ethyl 4-nitrobenzoate). The optical and antimicrobial properties of a complex were determined. The Ultraviolet-visible and Fourier-transform infra-red spectroscopy were used in monitoring optical activities of the resulting product. It was observed to absorb in the visible region (205 nm and 256 nm). The peaks...
Source: MethodsX - Category: Science Source Type: research
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Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
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Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
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Source: Toxicology Reports - Category: Toxicology Source Type: research
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Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
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Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
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