Low-Density Neuronal Cultures from Human Induced Pluripotent Stem Cells

We describe a differentiation protocol to generate low-density neuronal cultures ( ∼2,500 neurons/cm2) from human iPSC-derived neural stem cells/early neural progenitor cells. We generated low-density cultures using cells from 3 individuals. We also evaluated the morphometric features of neurons derived from 2 of these individuals, one harboring a microdeletion on chromosome 15q11.2 and the other without the microdeletion. An approximately 7.5-fold increase in the density of dendritic filopodia was observed in the neurons with the microdeletion, consistent with previous reports. Low-density neuronal cultures enable facile and unbiased comparisons of iPSC-derived neurons from different individuals or clones.Mol Neuropsychiatry 2017;3:28-36
Source: Molecular Neuropsychiatry - Category: Neuroscience Source Type: research