Differing molecular response of young and advanced maternal age human oocytes to IVM

AbstractSTUDY QUESTIONWhat effect does maternal age have on the human oocyte's molecular response toin vitro oocyte maturation?SUMMARY ANSWERAlthough polyadenylated transcript abundance is similar between young and advanced maternal age (AMA) germinal vesicle (GV) oocytes, metaphase II (MII) oocytes exhibit a divergent transcriptome resulting from a differential response toin vitro oocyte maturation.WHAT IS KNOWN ALREADYMicroarray studies considering maternal age or maturation stage have shown that either of these factors will affect oocyte polyadenylated transcript abundance in human oocytes. However, studies considering both human oocyte age and multiple stages simultaneously are limited to a single study that examined transcript levels for two genes by qPCR. Thus, polyadenylated RNA sequencing (RNA-Seq) could provide novel insight into age-associated aberrations in gene expression in GV and MII oocytes.STUDY DESIGN, SIZE, DURATIONThe effect of maternal age (longitudinal analysis) on polyadenylated transcript abundance at different stages was analyzed by examining single GV and singlein vitro matured MII oocytes derived from five young (YNG;< 30 years; average age 26.8; range 20 –29) and five advanced maternal age (AMA; ≥40 years; average age 41.6 years; range 40–43 years) patients. Thus, a total of 10 YNG (5 GV and 5 MII) and 10 AMA (5 GV and 5 MII) oocytes were individually processed for RNA-Seq analysis.PARTICIPANTS/MATERIALS, SETTINGS, METHODSPatients undergoin...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research