Genome stability of bovine in vivo -conceived cleavage-stage embryos is higher compared to in vitro -produced embryos

AbstractSTUDY QUESTIONIs the rate and nature of chromosome instability (CIN) similar between bovinein vivo-derived andin vitro-cultured cleavage-stage embryos?SUMMARY ANSWERThere is a major difference regarding chromosome stability ofin vivo-derived and invitro-cultured embryos, as CIN is significantly lower inin vivo-derived cleavage-stage embryos compared toin vitro-cultured embryos.WHAT IS KNOWN ALREADYCIN is common duringin vitro embryogenesis and is associated with early embryonic loss in humans, but the stability ofin vivo-conceived cleavage-stage embryos remains largely unknown.STUDY DESIGN, SIZE, DURATIONBecause humanin vivo preimplantation embryos are not accessible, bovine (Bos taurus) embryos were used to study CINin vivo. Five young, healthy, cycling Holstein Friesian heifers were used to analyze single blastomeres ofin vivo embryos,in vitro embryos produced by ovum pick up with ovarian stimulation (OPU-IVF), andin vitro embryos produced fromin vitro matured oocytes retrieved without ovarian stimulation (IVM-IVF).PARTICIPANTS/MATERIALS, SETTING, METHODSSingle blastomeres were isolated from embryos, whole-genome amplified and hybridized on Illumina BovineHD BeadChip arrays together with the bulk DNA from the donor cows (mothers) and the bull (father). DNA was also obtained from the parents of the bull and from the parents of the cows (paternal and maternal grandparents, respectively). Subsequently, genome-wide haplotyping and copy-number profiling was applied to in...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research