N-cadherin identifies human endometrial epithelial progenitor cells by in vitro stem cell assays

AbstractSTUDY QUESTIONIs there a specific surface marker that identifies human endometrial epithelial progenitor cells with adult stem cell activity usingin vitro assays?SUMMARY ANSWERN-cadherin isolates clonogenic, self-renewing human endometrial epithelial progenitor cells with high proliferative potential that differentiate into cytokeratin+ gland-like structuresin vitro and identifies their location in some cells of gland profiles predominantly in basalis endometrium adjacent to the myometrium.WHAT IS KNOWN ALREADYHuman endometrium contains a small population of clonogenic, self-renewing epithelial cells with high proliferative potential that differentiate into large gland-like structures, but their identity and location is unknown. Stage-specific embryonic antigen-1 (SSEA-1) distinguishes the epithelium of basalis from functionalis and is a marker of human post-menopausal (Post-M) endometrial epithelium.STUDY DESIGN, SIZE, DURATIONProspective observational study of endometrial epithelial cells obtained from hysterectomy samples taken from 50 pre-menopausal (Pre-M) and 24 Post-M women, of which 4 were from women who had taken daily estradiol valerate 2 mg/day for 8 weeks prior.PARTICIPANTS/MATERIALS, SETTING, METHODSGene profiling was used to identify differentially expressed surface markers between fresh EpCAM (Epithelial Cell Adhesion Molecule)-magnetic bead-selected basalis-like epithelial cells from Post-M endometrium compared with predominantly functionalis epithelia...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research

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Authors: Cetintas S, Tezcan G, Tunca B, Egeli U, Gokgoz MS, Cecener G Abstract Distant organ tumor dissemination is a major cause of breast cancer-related deaths. In 2010, we analyzed the prognostic importance of the circulating tumor markers (CTM) cytokeratin (CK)19, CK20, epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 2 (HER2) in relation to the clinical and pathological characteristics of patients with breast cancer (BC). To assess the clinical utility of CK19, CK20 and EGFR in predicting distant metastasis and recurrence in BC, here we report 7-year follow-up results of 77 pa...
Source: Bosnian Journal of Basic Medical Sciences - Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research
This article provides an overview of currently available relevant real world data sources and the utilization potential. It lists exemplary results of existing studies: secondary data analyses based on billing data from statutory health insurance funds were used among other things to estimate the participation rate in the statutory skin cancer screening. This was approximately 31% in the 2‑year period and was higher in the western German federal states than in the eastern ones. Furthermore, participation was higher for women than for men. Regional data in combination with sociostructural and sociodemographic indicators a...
Source: Der Hautarzt: Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete - Category: Dermatology Tags: Hautarzt Source Type: research
CONCLUSIONS: TRKA expression can be found in 1.6% of solid tumours and can be paralleled by NTRK1 gene rearrangements or mostly GCN gain. The prognostic and translational therapeutic impact of the latter remains to be established. PMID: 29802225 [PubMed - indexed for MEDLINE]
Source: Clinical Lung Cancer - Category: Cancer & Oncology Authors: Tags: J Clin Pathol Source Type: research
This study is based on 5,174 men aged 50-66 years, who in 1995-1996 participated in the first round of the Göteborg randomized screening trial (initial T-PSA level
Source: Scandinavian Journal of Urology - Category: Urology & Nephrology Tags: Scand J Urol Source Type: research
CONCLUSION: These findings support potential mechanism of delayed recovery after circadian disruption in bipolar animal model could be an alteration of relative PKC activities among mood regulation related brain regions. It is required to investigate the PKC downstream signaling related to the delayed recovery pattern. PMID: 30235919 [PubMed]
Source: Psychiatry Investigation - Category: Psychiatry Tags: Psychiatry Investig Source Type: research
CONCLUSION: Adiposity may have primed the carcinogenic potential, leading to lorazepam-associated carcinogenesis in overweight patients. Epidemiological studies addressing this issue should consider the potential modulator contributing to benzodiazepine-associated carcinogenesis. PMID: 30235918 [PubMed]
Source: Psychiatry Investigation - Category: Psychiatry Tags: Psychiatry Investig Source Type: research
CONCLUSION: This study helps identify the specific interaction surfaces involved in the pathologic phenotype of TR mutants and TRIAC as a possible therapeutic agent for patients suffering from RTH syndromes. PMID: 30235988 [PubMed - as supplied by publisher]
Source: Thyroid : official journal of the American Thyroid Association - Category: Endocrinology Tags: Thyroid Source Type: research
CONCLUSIONS: At a high-volume center, gasless transaxillary endoscopic thyroid surgery done with robotic assistance was a safe, efficient and effective approach in a diverse North American patient population. PMID: 30235982 [PubMed - as supplied by publisher]
Source: Thyroid : official journal of the American Thyroid Association - Category: Endocrinology Tags: Thyroid Source Type: research
Estradiol vaginal inserts (4 µg and 10 µg) for treating moderate to severe vulvar and vaginal atrophy: a review of phase 3 safety, efficacy and pharmacokinetic data. Curr Med Res Opin. 2018 Sep 21;:1-17 Authors: Constantine GD, Simon JA, Pickar JH, Archer DF, Bernick B, Graham S, Mirkin S Abstract OBJECTIVE: To review safety, efficacy, and pharmacokinetic (PK) data from the phase 3 REJOICE trial, which evaluated a 17ß-estradiol (E2) softgel vaginal insert approved in 2018 for moderate to severe dyspareunia associated with menopausal vulvar and vaginal atrophy (VVA). METHODS: REJOI...
Source: Current Medical Research and Opinion - Category: Research Tags: Curr Med Res Opin Source Type: research
In this study, we compared the osteoinductive effects of BMP-2/7 heterodimer in human adipose-derived stem cells (hASCs) with their homodimers BMP-2 and BMP-7, in which MC3T3-E1 cells were utilized as a positive control. The results indicated that BMP-2/7 was not a stronger inducer during the osteogenic differentiation of hASCs as that for MC3T3-E1, and extracellular-signal-regulated kinase signaling played a role in the different effects of BMP-2/7 between hASCs and MC3T3-E1. Our study demonstrates the osteoinductive effects of heterodimeric BMP-2/7 present in a cell-specific pattern and cautions should be taken when appl...
Source: Growth Factors - Category: Research Tags: Growth Factors Source Type: research
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