Adenovirus-mediated artificial microRNA targeting fibrinogen-like protein 2 attenuates the severity of acute pancreatitis in mice.

Adenovirus-mediated artificial microRNA targeting fibrinogen-like protein 2 attenuates the severity of acute pancreatitis in mice. Biosci Rep. 2017 Oct 20;: Authors: Ye X, Ding J, Chen Y, Dong J Abstract Severe acute pancreatitis (SAP) remains to be challenging for its unpredictable inflammatory progression from acute pancreatitis to SAP. Apoptosis is an important pathology of SAP. Fibrinogen-like protein 2 (FGL2) has been reported to be involved in apoptosis. The present study aimed to explore the therapeutic effect of an adenovirus-mediated artificial microRNA targeting FGL2 (Ad-FGL2-miRNA) in taurocholate-induced murine pancreatitis models. Sodium taurocholate was retrogradely injected into the biliopancreatic ducts of the C57/BL mice to induce SAP. FGL2 expression was measured with reverse transcription-PCR, western blotting and immunohistochemical staining. ELISA was used to detect the activity of amylase and the concentrations of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β). In addition, the mRNA levels of TNF-α and IL-1β were also detected. Finally, apoptosis was assessed by TUNEL method and western blotting. Ad-FGL2-miRNA significantly suppressed FGL2 expression and alleviated pancreatic injury. Also, Ad-FGL2-miRNA markedly inhibited a post-SAP increase in the activation of TNF-α and IL-1β. Finally, pretreatment with Ad-FGL2-miRNA ameliorated apoptosis at the early stage of SAP by modulating cleaved casp...
Source: Bioscience Reports - Category: Biomedical Science Authors: Tags: Biosci Rep Source Type: research