Calibration and analysis of a multimodal micro-CT and structured light imaging system for the evaluation of excised breast tissue.

Calibration and analysis of a multimodal micro-CT and structured light imaging system for the evaluation of excised breast tissue. Phys Med Biol. 2017 Oct 19;: Authors: McClatchy Iii DM, Rizzo EJ, Meganck J, Kempner J, Vicory J, Wells WA, Paulsen KD, Pogue BW Abstract A multimodal micro-computed tomography (CT) and multi-spectral structured light imaging (SLI) system is introduced and systematically analyzed to test its feasibility to aid in margin delineation during breast conserving surgery (BCS). Phantom analysis of the micro-CT yielded a signal-to-noise ratio (SNR) of 34, a contrast of 1.64, and a minimum detectable resolution of 240 m for a 1.2 min scan. The SLI system, spanning wavelengths 490 nm to 800 nm and spatial frequencies up to 1.37 mm-1, was evaluated with aqueous tissue simulating phantoms having variations in particle size distribution, scatter density, and blood volume fraction. The reduced scattering coefficient, μ's and phase function parameter, γ, were accurately recovered over all wavelengths independent of blood volume fractions from 0% to 4%, assuming a flat sample geometry perpendicular to the imaging plane. The resolution of the optical system was tested with a step phantom, from which the modulation transfer function (MTF) was calculated yielding a maximum resolution of 3.78 cycles per mm. The three dimensional (3D) spatial co-registration between the CT and optical imaging space was tested and shown to b...
Source: Physics in Medicine and Biology - Category: Physics Authors: Tags: Phys Med Biol Source Type: research