Plasmodium detection and differentiation by direct-on-blood PCR Nucleic Acid Lateral Flow Immunoassay (db-PCR-NALFIA): development, validation, and evaluation
Declining malaria transmission warrants the search for highly sensitive point-of-care diagnostics, especially in resource-limiting settings. Db-PCR-NALFIA is a simplified PCR-based technique with a lateral flow readout that does not require any sample preparation. Two duplex db-PCR-NALFIAs were developed: a pan-Plasmodium/P. falciparum and a pan-Plasmodium/P. vivax assay. Confirmed positive samples from returning travelers (n=61) and negative controls (n=40) were used for laboratory validations.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Johanna M. Roth, Laura de Bes, Patrick Sawa, George Omweri, Victor Osoti, Boris Oberheitmann, Henk D.F.H. Schallig, P ètra F. Mens Tags: Regular Article Source Type: research