Multiple roles of two efflux pumps in a polycyclic aromatic hydrocarbon-degrading Pseudomonas putida strain B6-2 (DSM 28064).

This study provides insights into the versatile physiological functions of the widely distributed efflux pumps in the biodegradation of PAHs.IMPORTANCE PAHs are notorious for their recalcitrance to degradation in environment. The high frequency of the occurrence of the efflux pump genes was observed in the genomes of effective PAH-degraders; however, their specific roles in the degradation of PAHs are still obscure. The significance of our study is identifying the function and mechanism of the efflux pump SrpABC of Pseudomonas putida strain B6-2 (DSM 28064) in the biphenyl degradation process. SrpABC is crucial in releasing the toxicity caused by intermediates that are unavoidably produced in PAH degradation, which will allow an understanding on how cells maintain the intracellular balance of materials. It is reasonable to suggest that this study will provide a new perspective on the PAH recalcitrance, and will shed light on enhancing the PAH degradation by genetic engineering.
PMID: 29030440 [PubMed - as supplied by publisher]

Source: Applied and Environmental Microbiology - October 13, 2017 Category: Microbiology Authors: Yao X, Tao F, Zhang K, Tang H, Xu P Tags: Appl Environ Microbiol Source Type: research