Purification, characterization and heterologous production of plantaricyclin A, a novel circular bacteriocin produced by Lactobacillus plantarum NI326.

Purification, characterization and heterologous production of plantaricyclin A, a novel circular bacteriocin produced by Lactobacillus plantarum NI326. Appl Environ Microbiol. 2017 Oct 13;: Authors: Borrero J, Kelly E, O'Connor PM, Kelleher P, Scully C, Cotter PD, Mahony J, van Sinderen D Abstract Bacteriocins from lactic acid bacteria (LAB) are of increasing interest in recent years due to their potential as natural preservatives against food and beverage spoilage microorganisms. In a screening study for LAB, we isolated a strain, Lactobacillus plantarum NI326, from olives with activity against a strain belonging to the beverage-spoilage bacterium Alicyclobacillus acidoterrestris spp. Genome sequencing of the strain enabled the identification of a gene cluster encoding a putative circular bacteriocin and proteins involved in its modification, transport and immunity. This novel bacteriocin, named plantaricyclin A (PlcA), was grouped into the circular bacteriocin subgroup II due to its high degree of similarity with other gassericin A-like bacteriocins. Purification of the supernatant of Lb. plantarum NI326 resulted in an active peptide with a molecular mass of 5,570 Da, corresponding to that predicted from the (processed) PlcA amino acid sequence. The Plc gene cluster was subsequently cloned and expressed in L. lactis NZ9000, resulting in the production of an active 5,570 Da bacteriocin in the supernatant. PlcA is produced as a 91-am...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research