Co-immunoprecipitation for Identifying Protein-Protein Interactions in Borrelia burgdorferi.

Co-immunoprecipitation for Identifying Protein-Protein Interactions in Borrelia burgdorferi.
Methods Mol Biol. 2018;1690:47-55
Authors: Iqbal H, Akins DR, Kenedy MR
Abstract
Co-immunoprecipitation can be utilized to study protein-protein interactions from various environments, cell types, or tissues. Herein, we describe a co-immunoprecipitation protocol that can be used to examine protein complexes found in the pathogenic spirochete Borrelia burgdorferi. The method outlined here has successfully identified known and unknown members of borrelial protein complexes and is an efficient method for studying protein interactions in this pathogenic spirochete.
PMID: 29032535 [PubMed - in process]

Source: Mol Biol Cell - October 18, 2017 Category: Molecular Biology Authors: Iqbal H, Akins DR, Kenedy MR Tags: Methods Mol Biol Source Type: research