Recombinant E. coli Dualistic Role as an Antigen-adjuvant Delivery Vehicle for Oral Immunization.

Recombinant E. coli Dualistic Role as an Antigen-adjuvant Delivery Vehicle for Oral Immunization. Methods Mol Biol. 2018;1690:347-357 Authors: Gomes-Solecki M, Richer L Abstract Escherichia coli is the mainstay tool for fundamental microbiology research due to its ease of cultivation and safety. Auxotrophic strains of the K-12 and B lineages of E. coli are the organisms of choice to produce recombinant proteins. Components present in the cell envelope of bacteria are also potent immune modulators and have been used to develop adjuvants. We used live E. coli, after induction of recombinant protein expression, to develop a vehicle which has a dualistic function of producing vaccine while presenting itself as the adjuvant to deliver oral vaccines against a number of infectious diseases, including Lyme disease. Here, we give an example using E. coli expressing B. burgdorferi Outer Surface Protein A, which was proven effective in reducing B. burgdorferi burden in infected ticks after a 5-year field trial of a baited formulation containing this reservoir targeted vaccine. PMID: 29032558 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research

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CONCLUSIONS: Immunization with native antigens might not be sufficient to obtain complete protection to infection. Nonetheless we showed that non-infectious B. burgdorferi can be an effective carrier to deliver and elicit a specific host response to T. pallidum antigens to assess the efficacy of syphilis vaccine candidates. PMID: 30797635 [PubMed - as supplied by publisher]
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
In this study, bird species migrating through Bulgaria were investigated as carriers of zoonotic pathogens. In total, 706 birds belonging to 46 species were checked for the presence of various bacterial pathogens (Campylobacter, Yersin ia, Salmonella, Listeria, Escherichia coli, Staphylococcus aureus, Francisella tularensis, Coxiella burnetii, Borrelia burgdorferi, and Brucella spp.). From 673 birds we investigated fecal samples, from the remaining 33, blood samples. We detected Campylobacter 16S rDNA gene in 1.3% of birds, but no ne were of pathogenic Campylobacter jejuni and Campylobacter coli species. Escherichia coli 1...
Source: Canadian Journal of Microbiology - Category: Microbiology Authors: Source Type: research
Answer:Borreliaspp., relapsing fever group. Molecular studies are needed to definitively identify the causative agent. However, knowing the exposure history of the patient also helps narrow the differential. In this case, we later found out that the patient is from the northwestern part of North America, and therefore this is most likelyBorrelia hermsii,one of the tick-borne relapsing feverBorreliaspecies.The other tick-borneBorreliain North America areB. parkeriiandB. turicatae.These species are transmitted through the bite of a soft-bodied tick in the genusOrnithodoros,and are usually associated with exposure to'rustic's...
Source: Creepy Dreadful Wonderful Parasites - Category: Parasitology Source Type: blogs
The warmer weather of spring and summer means the start of tick and mosquito season and the diseases they transmit, including Lyme disease, Rocky Mountain spotted fever, West Nile and Zika. A new report from the Centers for Disease Control and Prevention has found that illnesses from mosquito, tick and flea bites more than tripled in […]Related:Feds crack down on e-liquid packaging that looks like candy, juice boxesCDC director asks that his $375,000 salary be cut after questions raised22 states now affected by dangerous outbreak of E. coli illness from romaine lettuce
Source: Washington Post: To Your Health - Category: Consumer Health News Source Type: news
In this study, the chimeric proteins (A/C-2, A/C-4 and A/C-7.1) consisting of B-cell epitopes of outer surface proteins OspA and OspC from Borrelia genospecies prevalent in Eastern Slovakia, were designed, over-expressed in E. coli, and used to detect specific anti-Borrelia antibodies in serologically characterized sera from patients with Lyme-like symptoms to evaluate their diagnostic potential. RESULTS: Results showed that chimeras vary in their immuno-reactivity when tested with human sera. Compared with the results obtained from a two-tier test, the application of recombinant multi-epitope chimeric proteins as dia...
Source: Annals of Agricultural and Environmental Medicine : AAEM - Category: Environmental Health Authors: Tags: Ann Agric Environ Med Source Type: research
ConclusionsThere was a very high prevalence of D. immitis, hookworm and external parasites in Samoan dogs, and prophylactic medication is rarely administered. This is the first report confirming A. platys in Samoa and the South Pacific islands. The public health implications of poor management of the dogs should be considered and investigated further.
Source: Australian Veterinary Journal - Category: Veterinary Research Authors: Tags: SMALL ANIMALS Source Type: research
Conclusions: The results of detection and attribution studies can inform evidence-based risk management to reduce current, and plan for future, changes in health risks associated with climate change. Gaining a better understanding of the size, timing, and distribution of the climate change burden of disease and injury requires reliable long-term data sets, more knowledge about the factors that confound and modify the effects of climate on health, and refinement of analytic techniques for detection and attribution. At the same time, significant advances are possible in the absence of complete data and statistical certainty:...
Source: EHP Research - Category: Environmental Health Authors: Tags: Commentary Source Type: research
UggBeen trying to stamp out the awful reporting on the poop doping claims of Dr. Lauren Peterson. SeeIrresponsible reporting on "poop doping" from the Washington PostKudos to Bicycling Magazine for pedaling so so so much overselling of the microbiomeBut the crap keeps flowing. Here is the last - in the NY Post: Poop transplants are the final frontier in athletic doping | New York PostHere are some quotes from the story and my comments about them."The treatment helped her battle Lyme Disease, however, there was a downside."No evidence exists that this treatment helped her battle Lyme disea...
Source: The Tree of Life - Category: Microbiology Authors: Source Type: blogs
Conclusions:Zika can induce GBS after a brief viral illness. Neuropathy can be a mixture of demyelinating and axonal. Dysautonomia may also be present including severe dysphagia, orthostatics and retention.Zika RNA has a short detection window in serum and CSF making a diagnosis time sensitive. Serological assays using IgM and IgG cross-react with other flaviviruses making them unable to differentiate between recent Zika exposure and cross reactivity with Dengue and Chikungunya. Our pt likely had prior exposure to Dengue, being from an endemic area. Prior Dengue exposure may possibly modulate the response to Zika via antib...
Source: Neurology - Category: Neurology Authors: Tags: Zika, Chikungunya, West Nile Virus, and Other Viral Infections I Source Type: research
Conclusions:Focal thickening and enhancement of affected cranial nerve is seen in the majority of pediatric ROCN. These findings are best seen with thin MRI cuts and gadolinium infusion. Recognizing this pattern is important in establishing the diagnosis.Disclosure: Dr. Alawadhi has nothing to disclose. Dr. Saint-Martin has nothing to disclose. Dr. Oskoui has nothing to disclose.
Source: Neurology - Category: Neurology Authors: Tags: Headache: Basic and Clinical Science Source Type: research
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