Effects of Trypanosoma brucei infection and treatment on haematoserological response of West African Dwarf sheep to Brucella abortus vaccine

This study aims to investigate the effects ofTrypanosoma brucei infection and treatment on haematoserological status of West African Dwarf sheep toBrucella abortus vaccine. Twenty-four adult WAD sheep of mixed sexes were randomly assigned into 6 groups of 4 each. They were either infected with 1  × 106 trypanosomes intravenously (groups 2 –6) or uninfected (group 1). The different groups were treated as follows: group 2 (positive control—7 mg/kg diminazene aceturate (DA)), group 3 (7 mg/kg DA and 5 mg/kg levamisole), group 4 (7 mg/kg DA and 50, 000 IU vitamin A), group 5 (7 mg/kg DA and 50, 000 IU vitamin A 3 weeks post i nfection), and group 6 (7 mg/kg DA and 5 mg/kg levamisole and 50, 000 IU vitamin A). All the animals were vaccinated withB. abortus S19 vaccine 2  weeks post infection (PI). Parameters assessed parasitaemia, total and differential white blood cell counts, and antibody titres toB.abortus antigen. Average prepatent period was 5  days. Trypanosomes were completely cleared from the bloodstream following treatment. There was no relapse parasitaemia recorded. By weeks one and two post infection, there was significant (P 
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

Related Links:

This study presents the first evidence that the transcriptional regulator VjbR has important function in B. canis. In addition, according to its reduced virulence and the protective immunity it induces in mice, it can be a potential live attenuated vaccine against B. canis.
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Designing a potent recombinant vaccine, using the appropriate subunits with the greatest effect on stimulating the immune system, especially in the case of intracellular pathogens such as gram negative Brucella M...
Source: BMC Veterinary Research - Category: Veterinary Research Authors: Tags: Research article Source Type: research
Abstract Brucellar spondylodiscitis, the most prevalent and significant osteoarticular presentation of human Brucellosis, is difficult to diagnose and usually yields irreversible neurologic deficits and spinal deformities. However, no animal models of Brucellar spondylodiscitis exist, allowing for preclinical investigations. The present study investigated whether intraosseous injection of attenuated Brucella melitensis vaccine into rabbits' lumbar vertebrae imitates the radiographic and histopathological characteristics of human Brucellar spondylodiscitis. Radiographic and histopathological analyses at 8 we...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
Abstract Brucella melitensis Rev.1 is the live attenuated Elberg-originated vaccine strain of the facultative intracellular Brucella species, and is widely used to control brucellosis in small ruminants. However, Rev.1 may cause abortions in small ruminants that have been vaccinated during the last trimester of gestation, it is pathogenic to humans, and it induces antibodies directed at the O-polysaccharide (O-PS) of the smooth lipopolysaccharide, thus making it difficult to distinguish between vaccinated and infected animals. Rough Brucella strains, which lack O-PS and are considered less pathogenic, have been in...
Source: International Journal of Medical Microbiology - Category: Microbiology Authors: Tags: Int J Med Microbiol Source Type: research
In this study, three Brucella abortus recombinant proteins, malate dehydrogenase (rMdh), outer membrane proteins (rOmp) 10 and 19 were loaded in mucoadhesive chitosan nanoparticles (CNs) and induction of mucosal and systemic immunity were investigated after intranasal immunization of BALB/c mice. These antigens were also coimmunized as cocktail (rCocktail) to evaluate multiple antigen specific vaccine candidates. At 6-weeks post-immunization (wpi), antigen specific total IgG was increased in all of the immunized groups, predominantly IgG1. In addition, spleenocyte from rMdh-, rOmp19-, and rCocktail-immunized groups signifi...
Source: International Journal of Medical Microbiology - Category: Microbiology Authors: Tags: Int J Med Microbiol Source Type: research
AbstractBrucellosis is an important zoonosis worldwide. In livestock, it frequently causes chronic disease with reproductive failures that contribute to production losses, and in humans, it causes an often-chronic febrile illness that is frequently underdiagnosed in many low- and middle-income countries, including India. India has one of the largest ruminant populations in the world, and brucellosis is endemic in the country in both humans and animals. In November 2017, the International Livestock Research Institute invited experts from government, national research institutes, universities, and different international org...
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research
Publication date: Available online 4 October 2019Source: Veterinary MicrobiologyAuthor(s): Neeta Jain-Gupta, Steven G. Waldrop, Nancy M. Tenpenny, Sharon G. Witonsky, Stephen M. Boyle, Nammalwar SriranganathanAbstractBrucellosis is one of the most common zoonotic diseases worldwide. Almost 500,000 new human cases occur each year; yet there is no vaccine for human use. Moreover, there is no universal Brucella vaccine that would provide protection against all pathogenic species of Brucella. We generated a rough, live-attenuated B. neotomae strain by deleting the wboA gene encoding a glycosyltransferase. This strain lacks the...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
This study aimed to assess the protective efficacy of a novel Brucella vaccine formulation in goats. Twenty black goats were separated into 2 groups. Group A was injected with 3.0 × 109 CFU (colony-forming units)/mL of a Salmonella-based delivery system harboring only vector (pMMP65). Group B was immunized with 3.0 × 109 CFU/mL of the vaccine, a mixture of 3 Brucella vaccine strains (using a Salmonella-based delivery system) expressing each recombinant B. abortus Omp3b, BCSP31, and SOD protein. No Salmonella delivery strain was isolated from all tested lymph nodes and parenchymal organs. Serum immunoglobulin (I...
Source: Canadian Journal of Veterinary Research - Category: Veterinary Research Authors: Tags: Can J Vet Res Source Type: research
Publication date: Available online 18 September 2019Source: Molecular and Cellular ProbesAuthor(s): Lide Qin, Wenlong Nan, Yong Wang, Yueyong Zhang, Pengfei Tan, Yuqi Chen, Kairong Mao, Yiping ChenAbstractBrucella, the etiological agent of brucellosis, is an important zoonosis pathogen worldwide. Brucella infects humans and various domestic and wild animals, and represents a great threat to public health and animal husbandry. In the present study, we developed a real-time recombinase polymerase amplification (RPA) assay for the detection of Brucella. The assay targeted the bcsp31 gene of Brucella, and an RPA exo probe and ...
Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research
Brucellosis is one of the most prevalent bacterial zoonosis of worldwide distribution. The disease is caused by Brucella spp., facultative intracellular pathogens. Brucellosis in animals results in abortion of fetuses, while in humans, it frequently manifests flu-like symptoms and a typical undulant fever, being osteoarthritis a common complication of the chronic infection. The two most common ways to acquire the infection in humans are through the ingestion of contaminated dairy products or by inhalation of contaminated aerosols. Brucella spp. enter the body mainly through the gastrointestinal and respiratory mucosa; howe...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
More News: African Health | Brucellosis | Parasitology | Pathology | Study | Vaccines | Veterinary Vaccinations | Vitamin A | Vitamins