Different expression systems resulted in varied binding properties of anti-paclitaxel single-chain variable fragment antibody clone 1C2

AbstractThe binding properties of recombinant antibody fragments, such as affinity and specificity, determine their usefulness for therapeutic and analytical applications. Anti-paclitaxel single-chain variable fragment clone 1C2 (anti-PT scFv1C2) was expressed usingEscherichia coli cell andBombyx mori larvae expression systems. The binding characteristics of the scFvs were evaluated using indirect competitive ELISA. The linear range of binding between anti-PT scFv1C2 and paclitaxel was significantly different between the anti-PT scFv1C2s derived fromE. coli (1.056 –5.700 μg/ml) andB. mori (7.813 –1000 ng/ml), which indicated that different expression systems resulted in different sensitivities for paclitaxel determination. In addition, the binding specificity of anti-PT scFv1C2 varied between expression systems. This finding implied that the expression system significantly affects the bi nding properties of recombinant antibodies, especially antibodies against low-molecular-weight targets.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research