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Commentary: Comparative Analysis of Phylogenetic Assignment of Human and Avian ExPEC and Fecal Commensal Escherichia coli Using the (Previous and Revised) Clermont Phylogenetic Typing Methods and its Impact on Avian Pathogenic Escherichia coli (APEC) Classification

Marjanca Star čič Erjavec, Luka Predojević, Darja Žgur-Bertok
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Publication date: December 2017 Source:Microbial Pathogenesis, Volume 113 Author(s): Xiaoka Wu, Xiaolong Lv, Jinye Lu, Shengqing Yu, Yawei Jin, Jiangang Hu, Jiakun Zuo, Rongsheng Mi, Yan Huang, Kezong Qi, Zhaoguo Chen, Xiangan Han The LuxS/AI-2 quorum sensing mechanism can regulate the physiological functions of avian pathogenic Escherichia coli (APEC) through internalization of the small molecule autoinducer-2 (AI-2). The ptsI gene encodes enzyme I, which participates in the phosphotransferase system (PTS) that regulates the virulence and AI-2 internalization of bacteria. The aim of the present study was to determine the...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Publication date: December 2017 Source:Journal of Global Antimicrobial Resistance, Volume 11 Author(s): Mariya Azam, Iqra Ehsan, Sajjad-ur-Rahman, Muhammad Kashif Saleemi, Muhammad Rizwan Javed, Mashkoor Mohsin
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
In conclusion, induction of cytokine and antimicrobial peptides expression in intestinal microenvironments may provide a means of reducing C. jejuni colonization in broiler chickens, a key step in reducing the incidence of campylobacteriosis in humans.
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
AbstractCampylobacteriosis is a deadly disease which has developed resistance to most of the available chemotherapeutic agents. Although various studies provide evidence of acquired immunity following exposure toCampylobacter jejuni, no effective vaccine has been developed, still. Hence, there is an urgent need to identify potential vaccine candidates forCampylobacter species. In the proposed study,Campylobacter jejuni subsp.jejuni serotype O:2 (strain NCTC 11168) was taken and computational approach was employed to screenC. jejuni genome for promising vaccine candidates. From 1623 protein-coding sequences, 37 potential an...
Source: Interdisciplinary Sciences, Computational Life Sciences - Category: Bioinformatics Source Type: research
In this study, infection experiments in vivo and in vitro demonstrated that the adhesion of Escherichia coli (E. coli) to host cells significantly increased after H9N2 virus infection, and this increase was not caused by pathological damages. Subsequently, we constructed a late chicken embryo infection model and used proteomics techniques to analyze the expression of proteins associated with bacterial adhesion after H9N2 virus infection. A total of 279 significantly differential expressed proteins were detected through isobaric tags for relative and absolute quantitation (iTRAQ) coupled with nano-liquid chromatography-tand...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Rong Li, Mengjiao Guo, Jing Lin, Tongjie Chai, Liangmeng Wei
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study suggests that different AMC pathways affect the pathogenesis of APEC.
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Abstract The hypervariable antigenicity and immunosuppressive features of avian leukosis virus subgroup J (ALV-J) has led to great challenges to develop effective vaccines. Epitope vaccine will be a perspective trend. Previously, we identified a variant antigenic neutralizing epitope in hypervariable region 1 (hr1) of ALV-J, N-LRDFIA/E/TKWKS/GDDL/HLIRPYVNQS-C. BLAST analysis showed that the mutation of A, E, T and H in this epitope cover 79% of all ALV-J strains. Base on this data, we designed a multi-variant epitope ensemble vaccine comprising the four mutation variants linked with glycine and serine. The recombi...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
Thermophilic Campylobacter spp. and Salmonella spp. are zoonotic bacteria, commonly harbored in the enteric tract of avian species. This survey aimed at verifying the presence of these microorganisms in a heterogeneous Italian population of pet birds and captive birds of prey (e.g., Passeriformes, Psittaciformes, Accipitriformes, Falconiformes, Strigiformes) that live in close contact with humans. One hundred and fifty-one individuals were tested for thermophilic Campylobacter spp. and 444 for Salmonella spp.
Source: Journal of Exotic Pet Medicine - Category: Veterinary Research Authors: Source Type: research
Publication date: November 2017 Source:Antiviral Research, Volume 147 Author(s): Fei Yuan, Ying Chu, Lihong Qi, Hongmei Li, Shuhong Sun, Peng Zhao, Shuang Chang, Huijun Guo The present study is focused on investigating the immunoprotective effects of CpG-ODN/Poly(I:C) combined with the viral glycoprotein gp90 protein against reticuloendotheliosis virus (REV) infection in chickens. REV's gp90 gene was amplified from the REV-infected cells and expressed in Escherichia coli (E.coli). The expressed products, upon purification, were inoculated into 7-day-old chickens with PBS, CpG-ODN or Poly(I:C) adjuvant; Two booster inocula...
Source: Antiviral Therapy - Category: Virology Source Type: research
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