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Commentary: Comparative Analysis of Phylogenetic Assignment of Human and Avian ExPEC and Fecal Commensal Escherichia coli Using the (Previous and Revised) Clermont Phylogenetic Typing Methods and its Impact on Avian Pathogenic Escherichia coli (APEC) Classification

Marjanca Star čič Erjavec, Luka Predojević, Darja Žgur-Bertok
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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el S Abstract The pheno- and genotypic relatedness among Escherichia coli isolates from broilers with and without macroscopic lesions on the femoral head were investigated. In total, 219 isolates obtained from the bone marrow were characterized by serotyping, antimicrobial resistance profiles (AMR), phylogenetic grouping, detection of virulence-associated genes (VAG) and pulsed-field gel electrophoresis (PFGE). Serotyping revealed that 48.4% of the isolates were assigned to one of the three serotypes (O78:K80: 21.0%, O2:K1: 18.7%, O1:K1: 8.7%). Substantial phenotypic variation was also noticed in AMR testing as mo...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
This study might provide further information in the search for potential drug targets for prophylaxis of avian colibacillosis and for auxiliary antibiotics in the treatment of avian colibacillosis. In avian pathogenic Escherichia coli, exogenous AI‐2 increases the bacterial susceptibility to trimethoprim‐sulfamethoxazole (SXT) in a folate synthesis‐dependent pathway but not in the LsrR‐dependent manner.
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL RESEARCH Source Type: research
Abstract The aim of this study was to isolate and investigate the bacteriocinogenic and probiotic potential of new Gram-negative isolates. Of 22 bacterial isolates from pig intestine and chicken crops, ten isolates had demonstrated a good activity, and the most potent five strains were identified as four E. coli and one as Proteus sp. No virulence factors were detected for E. coli strains isolated from pig intestine. The semi-purified microcins proved to be resistant to temperature and pH variation, but sensitive to proteolytic enzymes. Of particular interest, strain E. coli P2C was the most potent, free of virule...
Source: Archives of Microbiology - Category: Microbiology Authors: Tags: Arch Microbiol Source Type: research
Abstract The emergence and spread of carbapenemase-producing Enterobacteriaceae (CPE) in wildlife and livestock animals poses an important safety concern for public health. With our in vivo broiler chicken infection study we investigated transfer and experimental microevolution of the blaNDM-1-carrying IncA/C2 plasmid (pRH-1238) introduced by avian native Salmonella (S.) Corvallis, without inducing antibiotic selection pressure. We evaluated dependency of the time point of inoculation on donor [S Corvallis (12-SA01738)] and a plasmid-free Salmonella spp. recipient [S Paratyphi B (dTa+), 13-SA01617] excretion by qu...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
Publication date: Available online 31 January 2018 Source:Veterinary Microbiology Author(s): Priscila Regina Guerra, Ana Herrero-Fresno, Susanne Elisabeth Pors, Shahana Ahmed, Dan Wang, Ida Thøfner, Fabio Antenucci, John Elmerdahl Olsen Over the last few years, polyamines have been described as key-signal of virulence in pathogenic bacteria. In the current study, we investigated whether the knockout of genes related to polyamine biosynthesis and putrescine transport affected the virulence of an avian pathogenic E. coli (APEC) strain. One-week-old White Leghorn chickens were infected intratracheally with mutants in ...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
In this study, the plasmid pSS28 harboring the APEC O2 O-antigen polysaccharide gene cluster (15.5 kb) was constructed. Biosynthesis of pSS28-encoded APEC O2 O-antigen in Salmonella vaccine strain S740 was validated by Western blot. The recombinant Salmonella vaccine strain S740 (pSS28) elicited homologous protection against virulent wild-type APEC O2 challenge in a chicken model. Furthermore, through equal-volume mixing the two monovalent vaccine strains S740 (pSS27) and S740 (pSS28), a bivalent vaccine candidate against both APEC O1 and O2 was developed. Immunization of chickens with the bivalent vaccine elicited produ...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
Targeted synthesis and characterization of a gene-cluster encoding NAD(P)H-dependent 3α-, 3β-, and 12α-hydroxysteroid dehydrogenases from Eggerthella CAG:298, a gut metagenomic sequence. Appl Environ Microbiol. 2018 Jan 12;: Authors: Mythen SM, Devendran S, Méndez-García C, Cann I, Ridlon JM Abstract Gut metagenomic sequences provide a rich source of microbial genes, the majority of which are annotated by homology or unknown. Genes and gene pathways that encode enzymes catalyzing biotransformation of host bile acids are important to identify in gut metagenomic sequences ...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
Complete sequence of rmtB-carrying IncI1 ST136 plasmid.Plasmid pEC008 harbored the blaTEM-1, rmtB, aacC2, tetA, folR, strAB genes, as well as a class 1 integron cassette array (|dfrA12|orfF|aadA2|), which were interspersed with different mobile elements, including Tn2, Tn1721, Tn1722, Tn5393, ISCfr1, IS5057, ISCR1, and ISCR2, and three copies of IS26. The MRR of pEC008 may have resulted from the acquisition of the Tn1722 transposition in the plasmid backbone.
Source: International Journal of Antimicrobial Agents - Category: Drugs & Pharmacology Authors: Tags: Short Communication Source Type: research
Publication date: December 2017 Source:Microbial Pathogenesis, Volume 113 Author(s): Xiaoka Wu, Xiaolong Lv, Jinye Lu, Shengqing Yu, Yawei Jin, Jiangang Hu, Jiakun Zuo, Rongsheng Mi, Yan Huang, Kezong Qi, Zhaoguo Chen, Xiangan Han The LuxS/AI-2 quorum sensing mechanism can regulate the physiological functions of avian pathogenic Escherichia coli (APEC) through internalization of the small molecule autoinducer-2 (AI-2). The ptsI gene encodes enzyme I, which participates in the phosphotransferase system (PTS) that regulates the virulence and AI-2 internalization of bacteria. The aim of the present study was to determine the...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Publication date: December 2017 Source:Journal of Global Antimicrobial Resistance, Volume 11 Author(s): Mariya Azam, Iqra Ehsan, Sajjad-ur-Rahman, Muhammad Kashif Saleemi, Muhammad Rizwan Javed, Mashkoor Mohsin
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
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