Biofilm formation by Candida albicans is inhibited by photodynamic antimicrobial chemotherapy (PACT), using chlorin e6: increase in both ROS production and membrane permeability

The objective of this study was to investigate the effects of PACT, using chlorin e6, as a photosensitizer onC. albicans. In this work, we studied the effect of PACT on both cell growth and biofilm formation byC. albicans. In addition, both ROS production and cell permeability were determined after PACT. PACT inhibited both growth and biofilm formation byC. albicans. We have also observed that PACT increased both ROS production (six times) and cell membrane permeability (five times) inC. albicans. PACT decreased both cell growth and biofilm development. The effect of PACT using chlorin e6 onC. albicans could be associated with an increase in ROS production, which could increase cell permeability, producing permanent damage to the cell membranes, leading to the cell death.
Source: Lasers in Medical Science - Category: Laser Surgery Source Type: research