Identification of novel antigens recognized by serum antibodies in bovine tuberculosis.

Identification of novel antigens recognized by serum antibodies in bovine tuberculosis. Clin Vaccine Immunol. 2017 Oct 04;: Authors: Lyashchenko KP, Grandison A, Keskinen K, Sikar-Gang A, Lambotte P, Esfandiari J, Ireton GC, Vallur A, Reed SG, Jones G, Vordermeier HM, Stabel JR, Thacker TC, Palmer MV, Waters WR Abstract Bovine tuberculosis (TB) caused by Mycobacterium bovis remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by multi-antigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in Dual-Path Platform (DPP®) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB. PMID: 28978510 [PubMed - as supplied by publisher]
Source: Clinical and Vaccine Immunology - Category: Allergy & Immunology Authors: Tags: Clin Vaccine Immunol Source Type: research