Antimicrobial Activities of Vernonia amygdalina Del and Prunus africana Extracts against Multidrug Resistant Clinical Strains

Background and Objective: Many plant-derived compounds have been used as drugs, either in their original or semi-synthetic forms. Plant derived metabolites can also serve as a lead compounds, which may be used as templates for the development of new drugs. Therefore, the aim of this study was to evaluate the antimicrobial activities of solvent fractions of Vernonia amygdalina shoot apex and Prunus africana bark against multidrug resistant bacterial strains. Materials and Methods: The plant materials were obtained through successive extractions using solvents of different polarity such as petroleum ether, chloroform, acetone and methanol. The antibacterial activities of the fractions were then evaluated by the hole-agar-well diffusion method. The minimum inhibitory concentration of the solvent fraction was determined against the isolated microorganism by agar dilution method. Then, the data were analyzed using Statistical Package for Social Science (SPSS) version 16. p
Source: Research Journal of Medicinal Plant - Category: Complementary Medicine Source Type: research

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ConclusionCoNS,S. aureus,E. coli andKlebsiella spp. were the leading bacterial causes of neonatal sepsis in our study. They were highly resistant to first- and second-line empiric antimicrobial treatment used at NICU (Neonatal intensive care unit), reducing the antimicrobial choices for management of neonatal sepsis. Fortunately, the mentioned isolated bacteria remained susceptible to third line antibiotics used to treat neonatal sepsis.
Source: Antimicrobial Resistance and Infection Control - Category: Infectious Diseases Source Type: research
Abstract Aim of this study was to evaluate polyphenols and major and trace elements of Cladanthus arabicus and Bubonium imbricatum, along with their in vitro antibacterial activity against six multidrug resistant Enterobacteriaceae (Escherichia coli S33/16, E. coli S34/16, Proteus mirabilis S32/16, Klebsiella pneumoniae S12/16, Enterobacter cloacae S5/16, and Salmonella sp S12/14). UV spectrophotometry, ultra-high-performance liquid chromatography coupled to mass spectrometry and inductively coupled plasma mass spectrometry were used to evaluate total polyphenol content, quali-quantitative profile of single polyph...
Source: Natural Product Research - Category: Biochemistry Authors: Tags: Nat Prod Res Source Type: research
Authors: Canlı K, Yetgin A, Benek A, Bozyel ME, Murat Altuner E Abstract The aim of this study was to test antimicrobial activity of ethanol extract of Lavandula stoechas against 22 bacteria and 1 yeast. Also, biochemical composition of the extract was investigated. A wide range of Gram-positive, Gram-negative microorganisms, and multidrug resistant bacteria were selected to test the antimicrobial activity. As a result, the extract is observed to contain fenchone (bicyclo[2.2.1]heptan-2-one, 1,3,3-trimethyl-, (1R)-) and camphor (+)-2-bornanone) as major components and showed antimicrobial activity against all stud...
Source: Advances in Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Adv Pharmacol Sci Source Type: research
Publication date: Available online 13 February 2019Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Aziz Ur Rahman, Arif Ullah Khan, Qipeng Yuan, Yun Wei, Aftab Ahmad, Sadiq Ullah, Zia Ul Haq Khan, Saira Shams, Muhammad Tariq, Waqas AhmadAbstractMetal nanoparticles, synthesized using Phyto-constituents are the most economically and environmentally benign materials ever. Biogenic silver nanoparticles (AgNPs) from three fractions of Arisaema flavum tuber extract were synthesized and characterized by UV–visible spectroscopy, XRD (X-rays diffraction), FT-IR (Fourier transform infrared spectroscopy)...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
In conclusion, this investigation illustrated simple green synthesis of ZnO NPs with suitable advantages of antioxidant, antibacterial, anti-biofilm, anti-QS activities, and also anti-swarming motility against MDR bacteria.Graphical abstract
Source: Journal of Industrial and Engineering Chemistry - Category: Chemistry Source Type: research
Abstract An increasing number of multidrug-resistant Acinetobacter baumannii (MDR-AB) infections have been reported worldwide, posing a threat to public health. The establishment of methods to elucidate the mechanism of action (MOA) of A. baumannii-specific antibiotics is needed to develop novel antimicrobial therapeutics with activity against MDR-AB We previously developed bacterial cytological profiling (BCP) to understand the MOA of compounds in E. coli and B. subtilis Given how distantly related A. baumannii is to these species, it was unclear to what extent it could be applied. Here we implemented bacterial c...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli are responsible for severe infections worldwide. Whereas their genotypic and pathogenic characteristics are not documented in Democratic Republic...
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Research article Source Type: research
The antibiotic trimethoprim is frequently used to manage Burkholderia infections, and members of the resistance-nodulation-division (RND) family of efflux pumps have been implicated in multidrug resistance of this species complex. We show here that a member of the distinct Escherichia coli multidrug resistance B (EmrB) family is a primary exporter of trimethoprim in Burkholderia thailandensis, as evidenced by increased trimethoprim sensitivity after inactivation of emrB, the gene that encodes EmrB. We also found that the emrB gene is up-regulated following the addition of gentamicin and that this up-regulation is due to re...
Source: Journal of Biological Chemistry - Category: Chemistry Authors: Tags: Microbiology Source Type: research
This study investigated the occurrence of antimicrobial-resistantEscherichia coli in dairy calves in southern Vietnam. Fecal samples were taken directly from the rectum of 84 calves from 41 smallholder dairy farms, when newborn and at 14  days of age for isolation ofE. coli. Escherichia coli strains were isolated from 144 of the 168 fecal samples tested. Of the 144E. coli isolates, 40% were found to be susceptible to all 12 antimicrobial drugs tested and 53% of theE. coli isolates were resistant to at least three antimicrobials. Calves were colonized with antimicrobial-resistantE. coli already on the day of birth. Res...
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research
Silver nanoparticles (AgNPs) are considered a good alternative for antibiotics due to emerging Multidrug Resistance (MDR) crisis. Resistance to AgNPs is approximately limited among Gram-positive and Gram-negative pathogens. Low toxicity to human cells permits its safe use as a new antimicrobial with broad spectrum. Bacterial cells are used as a factory for AgNPs synthesis supplying a powerfu...
Source: African Journal of Microbiology Research - Category: Microbiology Source Type: research
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