Comparison of SFRP2 promoter methylation in stool sample and cfDNA regarding patients with colorectal cancer
AbstractColorectal cancer (CRC) is one of the main causes of death in the world. Diagnosis in precancerous lesions stage is an important factor in reducing the mortality rate in CRC. Our study aimed at determining methylation status of secreted frizzled-related protein2 (SFRP2) gene in stool and serum samples of patients compared to healthy individuals. We collected serum and stool samples from 50 individuals including 25 patients with colorectal cancer and 25 healthy individuals. DNA was isolated from the serum and stool and treated by sodium bisulfite. It was then analyzed by methylation-specific polymerase chain reaction (MSP). Results showed that methylated SFRP2 occurs with significant different in patients with colorectal cancer compared to healthy individuals. Our result indicated that methylated SFRP2 occurs in 68% (17/25) and 48% (12/25) of stool DNA and cell-free DNA of patients with colorectal cancer, respectively. Methylated SFRP2 was detected in 8% (2/25) and 4% (1/25) of stool DNA and cfDNA of healthy individuals. Our result indicated that methylated SFRP2 occurred more frequently in stool DNA than cell-free DNA sample of patients with colorectal cancer. Methylated SFRP2 can be used as a potential noninvasive biomarker for screening high-risk individuals. In order to determine high sensitivity and specificity of this marker, more samples of Iranian individuals should be studied.
Authors: Liu X, Yang G, Huang J, Chai L, Liu X, Dai Q, Yang Z Abstract Aim: KRAS SNPs may increase KRAS transcription and KRAS levels. SNPs of KRAS 3'UTR can affect carcinoma risk and prognosis. Materials &methods: The rs8720 and rs7960917 in KRAS 3'UTR for colorectal carcinoma (CRC) risk and survival were investigated in a case-control study. Association between SNPs and CRC risk, survival analysis were analyzed by an unconditional logistic regression model, log-rank test, Kaplan-Meier estimation, Cox regression model and one-way analysis of variance. Results &conclusion: The genotype CT of rs8720 was sign...
Conclusion: These results suggest that miR-9-5p targeting of PAK4 could have therapeutic potential for CRC treatment. PMID: 31728150 [PubMed - in process]
ConclusionsPractical difficulties in quantifying quality of life losses and healthcare expenditures owing to competing risks in life years gained can be overcome. Their inclusion can have a substantial impact on the cost-effectiveness of cancer screening programs.
Abstract Cloning the open reading frame (ORF) of a protein-coding chimeric RNA into a mammalian expression vector is a simple, practiced way to study the function of the chimeric RNA. Here, we provide procedures for the insertion of the RRM2-C2orf48 fusion ORF into a mammalian expression vector, achieving the overexpression of the RRM2-C2orf48 fusion protein in a colon cancer cell line by retroviral transduction. PMID: 31728969 [PubMed - in process]
Authors: Colhado Rodrigues BL, Lallo MA, Perez EC Abstract Autophagy is a natural regulatory mechanism of the cell that eliminates unnecessary and dysfunctional cellular components to maintain homeostasis. Several authors have demonstrated that this mechanism can be induced by pathological conditions as cancer. However, their role in tumor development is still a controversial issue in cancer research. Here, we discussed the most relevant findings concerning autophagy in tumor development. In this critical review performed with studies published between 2002 and 2018, we found that the main pathway involved in the a...
AbstractBackgroundThe role of tumour-associated macrophages (TAMs) in predicting the prognosis of colorectal cancer (CRC) remains controversial. This is especially so because the prognostic significance and clinicopathological relevance of different subtypes of TAMs in the immune microenvironment of CRC have not yet been established.ObjectiveTo assess the clinicopathological and prognostic value of pan-macrophages, M1-macrophages or M2-macrophages in patients with CRC.MethodsComprehensive searched on the Medline/PubMed, Web of Science (WoS) and Google Scholar databases was conducted to identify relevant studies published u...
ConclusionAlthough classically, majority of studies have shown oncogenic roles of nucleoporins as genetic fusion partners in several types of leukemia, emerging evidence suggests that nucleoporins also modulate many cellular signaling pathways that are associated with several major non-hematological malignancies, such as carcinomas of skin, breast, lung, prostate and colon. Hence, nucleoporins are emerging as novel therapeutic targets in human tumors.
Conditions: Colon Cancer; Rectal Cancer; Frailty Intervention: Other: Prehabilitation Sponsor: Zealand University Hospital Recruiting
Condition: Colorectal Cancer Intervention: Drug: Temozolomide + Olaparib Sponsor: Yale University Not yet recruiting
Conditions: Metastatic Colorectal Cancer; Colorectal Neoplasms; Colorectal Carcinoma; Colorectal Cancer With Hepatic Metastases; Colorectal Tumors Interventions: Biological: VB-111; Drug: Nivolumab Sponsor: National Cancer Institute (NCI) Not yet recruiting