Dermal –epidermal separation methods: research implications

AbstractDermal –epidermal separation is an important basic investigation technique for pharmacology, toxicology and biology. To choose the optimal method for a given research need, we reviewed studies on epidermal separation. PubMed, Embase, and Web of Science were utilized. Different separation systems have bee n compared, including chemical, enzyme, heat and mechanical separation; each has advantages and disadvantages. Heat is simple but causes thermal damage. Chemical reagents are effective but disturb cellular electrolyte equilibrium. Enzymes provide complete separation, but destroy important components . Mechanical division does not alter physical and/or chemical integrity, but necessitates a relatively large sample and may cause cross-contamination of layers. Thus, the appropriate method should be chosen for a given research question, and no single method appears superior for all purposes.
Source: Archives of Dermatological Research - Category: Dermatology Source Type: research