Omics analyses of Trichoderma reesei CBS999.97 and QM6a indicate a relevance of female fertility for CAZyme and transporter levels.

Omics analyses of Trichoderma reesei CBS999.97 and QM6a indicate a relevance of female fertility for CAZyme and transporter levels. Appl Environ Microbiol. 2017 Sep 15;: Authors: Tisch D, Pomraning KR, Collett JR, Freitag M, Baker SE, Chen CL, Hsu PW, Chuang YC, Schuster A, Dattenböck C, Stappler E, Sulyok M, Böhmdorfer S, Oberlerchner J, Wang TF, Schmoll M Abstract The filamentous fungus Trichoderma reesei is predominantly found in the tropics, but also in more temperate regions, such as Europe and is widely known as producer of high amounts of plant cell wall degrading enzymes. We sequenced the genome of the sexually competent isolate CBS999.97, which is phenotypically different from female sterile QM6a, but can cross sexually with QM6a. Transcriptome data for growth on cellulose showed that entire CAZyme families are consistently differentially regulated between these strains. We evaluated backcrossed strains of both mating types, which acquired female fertility from CBS999.97, but maintained a mostly QM6a genetic background and could thereby distinguish between effects of strain background and female fertility or mating type. We found clear regulatory differences associated with female fertility and female sterility, that included CAZyme and transporter genes. Analysis of carbon source utilization, transcriptome and secondary metabolites in these strains revealed that only a few changes in gene regulation are consistently corre...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research