The influence of acidic media on the effect of beta-amyloid peptide on the function of glycine receptor in hippocampal neurons

Publication date: Available online 15 September 2017 Source:Neurochemistry International Author(s): J.V. Bukanova, E.I. Solntseva, R.V. Kondratenko, V.G. Skrebitsky We have previously shown that application of beta-amyloid peptide 1–42 (Aβ) at picomolar/nanomolar concentrations caused a decrease in the peak amplitude and acceleration of desensitization of the glycine-activated chloride current (I Gly) in hippocampal pyramidal neurons (Bukanova et al., 2016). The aim of this work was to study the effect of Aβ on I Gly in an acidified medium. The relevance of this work is determined by the fact that the pathogenic effects of Aβ in Alzheimer's disease are usually accompanied by inflammatory processes and acidosis. The I Gly was induced by 600 ms application of 100 μM (nearly EC50) or 500 μM (nearly saturating) glycine on isolated rat hippocampal neurons. The solution of glycine was neutral (pH 7.4) or acidic over a pH range of 5.0–7.0. It was found that 600 ms application of protons rapidly, reversibly and in dose-dependent manner decreased the peak amplitude and accelerated the desensitization of I Gly. The effect of H+ on I Gly desensitization did not depend on glycine concentration and may be considered noncompetitive, while the effect on I Gly peak disappeared at saturating glycine concentration and can be regarded as a competitive. These characteristics of the proton effects on I Gly coincide with the characteristics of the Aβ effects on I Gly. Experimen...
Source: Neurochemistry International - Category: Neuroscience Source Type: research