Chronic Global Analysis of Vascular Permeability and Cerebral Blood Flow after Bone Marrow Stromal Cell Treatment of Traumatic Brain Injury in the Rat: A long-term MRI Study.

Chronic Global Analysis of Vascular Permeability and Cerebral Blood Flow after Bone Marrow Stromal Cell Treatment of Traumatic Brain Injury in the Rat: A long-term MRI Study. Brain Res. 2017 Sep 09;: Authors: Li L, Chopp M, Ding G, Li Q, Mahmood A, Jiang Q Abstract Vascular permeability and hemodynamic alteration in response to the transplantation of human bone marrow stromal cells (hMSCs) after traumatic brain injury (TBI) were longitudinally investigated in non directly injured and normal-appearing cerebral tissue using magnetic resonance imaging (MRI). Male Wistar rats (300-350g, n=30) subjected to controlled cortical impact TBI were intravenously injected with 1 ml of saline (at 6-hours or 1-week post-injury, n=5/group) or with hMSCs in suspension (∼3x10(6) hMSCs, at 6-hours or 1-week post-injury, n=10/group). MRI measurements of T2-weighted imaging, cerebral blood flow (CBF) and blood-to-brain transfer constant (Ki) of gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA), and neurological behavioral estimates were performed on all animals at multiple time points up to 3-months post-injury. Our long-term imaging data show that blood-brain barrier (BBB) breakdown and hemodynamic disruption after TBI, as revealed by Ki and CBF, respectively, affect both hemispheres of the brain in a diffuse manner. Our data reveal a sensitive vascular permeability and hemodynamic reaction in response to the time-dependent transplantation of h...
Source: Brain Research - Category: Neurology Authors: Tags: Brain Res Source Type: research