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Occurrence of vancomycin-resistant Staphylococcus aureus in the oral cavity of patients with dental caries.

Occurrence of vancomycin-resistant Staphylococcus aureus in the oral cavity of patients with dental caries. Acta Microbiol Immunol Hung. 2017 Sep 11;:1-9 Authors: Vellappally S, Divakar DD, Al Kheraif AA, Ramakrishnaiah R, Alqahtani A, Dalati MHN, Anil S, Khan AA, Harikrishna Varma PR Abstract Oral streptococci are the major group of microbes isolated from oral microflora. They represent frequent pathogens of infective endocarditis (IE), and it is assumed that in most of the cases oral streptococci are acquired via mucosa layer of oral cavity. Staphylococcus aureus is also frequently isolated from IE as it accounts for 20%-30% of all cases. Vancomycin has been the most reliable therapeutic agent against infections caused by methicillin-resistant S. aureus (MRSA). The main objective of this study was to examine the occurrence of S. aureus species in dental caries specimens. Antimicrobial susceptibility testing of S. aureus to four antibiotics namely vancomycin, linezolid, teicoplanin, and daptomycin was performed. Detection of vancomycin resistance was conducted using polymerase chain reaction. Among the tested 150 strains, 98 were MRSA and of that 54 were vancomycin sensitive and 27 were resistant. All 98 MRSA strains were positive for mecA and 36 yielded pvl, whereas 13 carried vanA and only 2 were positive for vanB. Majority of the isolates showed sensitivity toward daptomycin and linezolid. Strains of S. aureus exhibiting decreased susceptibility to d...
Source: Acta Microbiologica et Immunologica Hungarica - Category: Microbiology Authors: Tags: Acta Microbiol Immunol Hung Source Type: research

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Org. Biomol. Chem., 2017, Accepted Manuscript DOI: 10.1039/C7OB02060K, PaperAndreas Kirschning, Thomas Schmidt, Jonas Ammermann, Irene Wagner-Dobler, Michael Reck, Meike Stiesch, Nico Stumpp, Marly Dalton, Jannik Donner Carolacton, a secondary metabolite isolated from the extracts of Sorangium cellulosum, causes membrane damage and cell death in biofilms of the caries- and endocarditis-associated bacterium Streptococcus mutans and Streptococcus pneumoniae.... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic and Biomolecular Chemistry - Category: Molecular Biology Authors: Source Type: research
Journal of Agricultural and Food ChemistryDOI: 10.1021/acs.jafc.7b02592
Source: Journal of Agricultural and Food Chemistry - Category: Food Science Authors: Source Type: research
In this study, we aimed to develop a direct method for accurate and rapid detection of MRSA in clinical samples from open environments, such as nasal swabs. The new molecular assay is based on detecting the cooccurrence of nuc and mecA markers in a single bacterial cell by utilizing droplet digital PCR (ddPCR) with the chimeric lysin ClyH for cell lysis. The method consists of (i) dispersion of an intact single bacterium into nanoliter droplets, (ii) temperature-controlled release of genomic DNA (gDNA) by ClyH at 37°C, and (iii) amplification and detection of the markers (nuc and mecA) using standard TaqMan chemistries...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Bacteriology Source Type: research
Disk diffusion and MIC quality control (QC) ranges were determined for nafithromycin, a new lactone-ketolide, following the completion of a nine-laboratory, Clinical and Laboratory Standards Institute (CLSI) document M23-defined tier 2 study. Five QC strains consistent with the spectrum of activity of nafithromycin were tested: Staphylococcus aureus ATCC 25923 (disk only), S. aureus ATCC 29213 (broth only), Enterococcus faecalis ATCC 29212 (broth only), Streptococcus pneumoniae ATCC 49619 (disk and broth), and Haemophilus influenzae ATCC 49247 (disk and broth). Nafithromycin disk diffusion QC ranges were determined to be 2...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Bacteriology Source Type: research
ConclusionsCVC salvage was feasible in the majority of children withS. aureus CLABSI and was not associated with significant complications or attributable mortality as reported in adults.
Source: Pediatric Surgery International - Category: Surgery Source Type: research
Abstract ObjectiveTo identify the impact of MUC5B polymorphisms on dental caries. MethodsA case‐control study was performed with patients recruited at Pontifícia Universidade Católica do Paraná. This individuals were aged 12 years old or more. Dental caries was diagnosed using the International Caries Detection and Assessment System and the effects of socioeconomic, dietary, and hygiene factors on dental caries were investigated. Furthermore, buccal cells were collected, and their DNA was extracted and amplified using PCR. Uni‐, bi‐, and multivariate analyses were performed. ResultsTwo‐hundred ...
Source: Oral Diseases - Category: ENT & OMF Authors: Tags: Original Article Source Type: research
Publication date: December 2017 Source:Wound Medicine, Volume 19 Author(s): Basavraj Nagoba, Milind Davane, Rajan Gandhi, Bharat Wadher, Namdev Suryawanshi, Sohan Selkar Multiple antibiotic resistant Pseudomonas aeruginosa is a significant cause of burn wound infections and, skin and soft tissue infections. Because of its resistance to commonly used antibiotics and antiseptics, there is a shortage of therapeutic options for effective treatment. The present review summarizes the use of citric acid as a most potential substitute for antiseptics to control pseudomonal wound infections and its role in the process of wound hea...
Source: Wound Medicine - Category: General Medicine Source Type: research
Publication date: 25 November 2017 Source:International Journal of Pharmaceutics, Volume 533, Issue 1 Author(s): Mahmoud E. Awad, Alberto López-Galindo, Massimo Setti, Mahmoud M. El-Rahmany, César Viseras Iborra Kaolinite Al2Si2O5(OH)4 is an abundant and inexpensive geomaterial regarded as one of the most common clay minerals in the earth's crust and the most widespread phase among the other kaolin polymorphs (halloysite, dickite and nacrite). Structurally, it is a hydrous aluminum phyllosilicate member belonging to the dioctahedral 1:1 kaolin mineral group. The particle size of the pseudohexagonal kaolinite...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
AbstractIn this article we report the first case of Q fever endocarditis in a 13  years old child with a percutaneous pulmonary Melody® valve. The patient had a new onset of Melody valve dysfunction associated with the combination of hepatosplenomegaly and pancytopenia but was afebrile. Although blood cultures were negative, we have further investigated in the direction of infective endocarditis by performing PCR detection and the serology ofC. burnetii which were positive. A combination antibiotic therapy with doxycycline and hydroxychloroquine was started with good clinical evolution. Our case emphasizes the fac...
Source: Pediatric Cardiology - Category: Cardiology Source Type: research
This study aims to determine the antimicrobial activity of VIO on S. aureus in planktonic culture and biofilms. VIO showed excellent antimicrobial activity in inhibiting and killing S. aureus in planktonic cultures and biofilm formation. The minimum bactericidal concentration (5 μg/mL) of VIO caused the death of S. aureus after 3–4 h of exposure and the minimum inhibitory concentration (1.25 μg/mL) of VIO inhibited bacterial growth within the first 8 h of contact. Biofilm formation was also strongly inhibited by VIO (1.25 μg/mL), in contrast to the higher resistance verified for S. aureus in mature biofilm (...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
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