High substrate uptake rates empower Vibrio natriegens as production host for industrial biotechnology.

High substrate uptake rates empower Vibrio natriegens as production host for industrial biotechnology. Appl Environ Microbiol. 2017 Sep 08;: Authors: Hoffart E, Grenz S, Lange J, Nitschel R, Müller F, Schwentner A, Feith A, Lenfers-Lücker M, Takors R, Blombach B Abstract The productivity of industrial fermentation processes is essentially limited by the biomass specific substrate consumption rate (qS) of the applied microbial production system. Since qS depends on the growth rate (μ), we highlight the potential of the fastest growing non-pathogenic bacterium, Vibrio natriegens, as novel candidate for future biotechnological processes. V. natriegens grows rapidly in BHIN complex medium with a μ of up to 4.43 h(-1) (doubling time of 9.4 min) as well as in minimal medium supplemented with various industrially relevant substrates. Bioreactor cultivations in minimal medium with glucose showed that V. natriegens possesses an exceptionally high qS under aerobic (3.90 ± 0.08 g g(-1) h(-1)) and anaerobic (7.81 ± 0.71 g g(-1) h(-1)) conditions. Fermentations with resting cells of genetically engineered V. natriegens under anaerobic conditions yielded an overall volumetric productivity of 0.56 ± 0.10 g alanine L(-1) min(-1) (i.e. 34 g L(-1) h(-1)). These inherent properties render V. natriegens a promising new microbial platform for future industrial fermentation processes operating with high productivity.Importance Low conversion rates ...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research