Semi-high-throughput detection of Plasmodium falciparum and Plasmodium vivax oocysts in mosquitoes using bead-beating followed by circumsporozoite ELISA and quantitative PCR

The malaria infection status of mosquitoes is commonly determined by microscopic detection of oocysts on the dissected mosquito midgut. This method is labour-intensive, does not allow processing of large numbe...

Source: Malaria Journal - September 6, 2017 Category: Infectious Diseases Authors: Wouter Graumans, Fitsum G. Tadesse, Chiara Andolina, Geert-Jan van Gemert, Karina Teelen, Kjerstin Lanke, Endalamaw Gadisa, Delenasaw Yewhalaw, Marga van de Vegte-Bolmer, Rianne Siebelink-Stoter, Isa ïe Reuling, Robert Sauerwein and Teun Bousema Tags: Methodology Source Type: research