Prevalence and molecular typing of methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin gene.

Prevalence and molecular typing of methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin gene. Acta Microbiol Immunol Hung. 2017 Sep 01;:1-14 Authors: Hesari MR, Salehzadeh A, Darsanaki RK Abstract Panton-Valentine leukocidin (pvl) toxin is an important virulence factor of Staphylococcus aureus. The main genes are coa and spa for distinguishing and typing of S. aureus isolates. The aim of this study was to investigate antibiotic resistance, presence of mecA and pvl genes, as well as epidemiological typing of these isolates according to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in clinical sample isolated from Rasht city, Iran. A total of 250 clinical samples have been isolated from different hospitals. First, isolates of S. aureus were identified through microbiological methods and their antibiotic sensitivity was determined by disk diffusion agar based on a standard method of Clinical and Laboratory Standards Institute. DNA was extracted by boiling and presence of pvl and mecA genes was investigated by PCR using specific primers. To type these isolates, amplification of fragments of coa and spa genes was done and restriction enzyme digestion pattern was determined by PCR-RFLP method. Among the 250 samples, 50 isolates belonged to S. aureus and results of antibiotic sensitivity showed that 68% (34 samples) of isolates were methicillin resistant. Frequency of mecA a...
Source: Acta Microbiologica et Immunologica Hungarica - Category: Microbiology Authors: Tags: Acta Microbiol Immunol Hung Source Type: research