Apoptosis induced by Moringa oleifera Lam. pod in mouse colon carcinoma model

AbstractThe aim of this study was to investigate the effect of boiledMoringa oleifera Lam. pod (bMO) in colon carcinogenesis via apoptotic pathway. Four groups of mice (eight mice/group) were induced by azoxymethane (AOM, 10  mg/kg BW i.p. at the third week) followed by dextran sodium sulfate (DSS) for 7 days (2% in drinking water at the fourth week), while one negative group (n = 8) received AIN-76A basal diet without AOM/DSS induction. Positive control group received basal diet with AOM/DSS induction, whereas treatment groups received basal diet containing 1.5%, 3.0%, and 6.0% of bMO at the 5th week till 20th week after AOM/DSS inductions. Mice were sacrificed and necr opsied at the end of the 20th week. The results showed tumor incidence 87.5% and multiplicity 9.5 in positive control group. Subsequently, mice that received 1.5%–6.0% bMO exhibited tumor incidences and multiplicities at 25%–75% and 1.8–8.3, respectively. Pro-apoptosis protein BAX investigated by immunohistochemistry in mice treated with 3.0% bMO was significantly higher than positive control. Anti-apoptosis protein BCL-2 showed the lowest expression in mice fed with 6.0% bMO. The BAX/BCL-2 ratio proteins and the nuclear staining were found at the highest rate in mice received 3.0% bMO. This finding suggested that the bMO exhibited potent apoptosis induction through dissimilarity of BCL-2 family protein expression, thereby decreased BCL-2 protein expression and increased ...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

Related Links:

Publication date: Available online 24 February 2020Source: Respiratory Medicine Case ReportsAuthor(s): Takayasu Ito, Shotaro Okachi, Tadasuke Ikenouchi, Futoshi Ushijima, Takamasa Ohashi, Masahiro Ogawa, Masato Nagahama, Keiko Wakahara, Naozumi HashimotoAbstractA 65-year-old man with chronic respiratory failure caused by chronic obstructive pulmonary disease, had a pulmonary nodule adjacent to the inlet of right B1 and B3. The patient had undergone a surgery for right renal cell carcinoma and colon cancer 6 years prior. We attempted endobronchial ultrasound-guided transbronchial needle aspiration under non-invasive positiv...
Source: Respiratory Medicine Case Reports - Category: Respiratory Medicine Source Type: research
ConclusionDespite malignant disease, overall survival of patients after metastasectomy for pancreatic metastasis is acceptable. Better survival appears to be associated with the primary tumor entity. Further research should focus on molecular markers to elucidate the mechanisms of pancreatic metastasis to choose the suitable therapeutic approach for the individual patient.
Source: Clinical and Experimental Metastasis - Category: Cancer & Oncology Source Type: research
Colonoscopy with polypectomy is frequently performed in pediatric patients based on symptoms, with the majority of polyps identified being benign juvenile pedunculated polyps with a vascular stalk. This is in distinction to adults where polypectomy is often performed as part of a colon cancer screening and prevention strategy and a higher fraction of polyps are sessile and or dysplastic. In adults, polypectomy techniques emphasize a need for deeper resection to ensure complete resection of adenomas or potential carcinoma in situ. Adenomatous polyps can occur in the pediatric age group and may be associated with an underlyi...
Source: Journal of Pediatric Gastroenterology and Nutrition - Category: Gastroenterology Tags: Topic of the Month Source Type: research
Authors: Melloul S, Mosnier JF, Masliah-Planchon J, Lepage C, Le Malicot K, Gornet JM, Edeline J, Dansette D, Texereau P, Delattre O, Laurent Puig P, Taieb J, J-F E Abstract SMARCB1 is a tumor suppressor gene, which is part of SWI/SNF complex involved in transcriptional regulation. Recently, loss of SMARCB1 expression has been reported in gastrointestinal carcinomas. Our purpose was to evaluate the incidence and prognostic value of SMARCB1 loss in colon carcinoma (CC). Patients with stage III CC (n= 1695), and a second cohort of 23 patients with poorly differentiated CC were analyzed. Immunohistochemistry for SMARC...
Source: Cancer Biomarkers - Category: Cancer & Oncology Tags: Cancer Biomark Source Type: research
Conclusions: In conclusion, we developed a novel six-miRNA-based model to predict 5-year survival probabilities for CA patients. This model has the potential to facilitate individualized treatment decisions.
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Molecules, Vol. 25, Pages 964: Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis‐ Inducing Effects on Human Leukemic Cells Molecules doi: 10.3390/molecules25040964 Authors: Koutova Kulhava Havelek Majorosova Královec Habartova Hošťálková Opletal Cahlikova Řezáčová Bersavine is the new bisbenzylisoquinoline alkaloid isolated from the Berberis vulgaris L.(Berberidaceae) plant. The results of cytotoxicity screening 48 h post‐treatment showed thatbersavine considerably inhibits the proliferatio...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
AbstractWith the great success of anti-CTLA-4 and anti-PD-1 therapeutics in cancer immunotherapy, tumor necrosis factor receptor superfamily members have been recognized as ideal targets to provide co-stimulatory signals in combination with immune checkpoint blocking antibodies. Among these is OX40 (CD134), a co-stimulatory molecule expressed by activated immune cells. Recently, several anti-OX40 agonistic monoclonal antibodies, pogalizumab as the most advanced, have entered early phase clinical trials. Using a  yeast platform and multiple screening methods, we identified a fully human anti-OX40 antibody (IBI101) with...
Source: Cancer Immunology, Immunotherapy - Category: Cancer & Oncology Source Type: research
letal Lucie Cahlikova Martina Řezáčová Bersavine is the new bisbenzylisoquinoline alkaloid isolated from the Berberis vulgaris L. (Berberidaceae) plant. The results of cytotoxicity screening 48 h post-treatment showed that bersavine considerably inhibits the proliferation and viability of leukemic (Jurkat, MOLT-4), colon (HT-29), cervix (HeLa) and breast (MCF-7) cancer cells with IC50 values ranging from 8.1 to 11 µM. The viability and proliferation of leukemic Jurkat and MOLT-4 cells were decreased after bersavine treatment in a time- and dose-dependent manner. Bersavine manifested concen...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Publication date: Available online 17 February 2020Source: International Journal of PharmaceuticsAuthor(s): Amin Afkhami-Poostchi, Mansour Mashreghi, Mehrdad Iranshahi, Maryam M. MatinAbstractBacteria-directed enzyme prodrug therapy (BDEPT), is an emerging alternative directed and tumor-specific approach. The basis of this method is the conversion of a non-toxic prodrug by a bacterial enzyme to a toxic drug within the tumor-microenvironment (TME). In the present study, the therapeutic efficacy of BDEPT was investigated based on the ability of Escherichia coli DH5α-lux/βG in activation of glycyrrhizic acid (GL), ...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
CONCLUSION: Therefore, apart from probable anti-cancerous effect of rTC-1 on C26-A and SW1353 cell lines, this peptide may be able to mimic the anti-cancerous pathway of IL-8. PMID: 32072885 [PubMed - as supplied by publisher]
Source: Protein and Peptide Letters - Category: Biochemistry Authors: Tags: Protein Pept Lett Source Type: research
More News: Cancer | Cancer & Oncology | Carcinoma | Colon Cancer | Colorectal Cancer | Diets | Moringa Oleifera | Nutrition | Pathology | Sodium | Study