Stroma-Free Long-Term Growth of Primary Acute Myeloid Leukemia (AML) Cells

To individualize therapy for relapsed/refractory AML patients, robust in-vitro culture conditions to support primary leukemic cells are essential for drug sensitivity testing. Our lab has validated a high throughput chemosensitivity assay with primary AML cells maintained by cytokines; however, cytokines have not been shown to support long-term assays of primary AML cells. Stromal cells of the tumor microenvironment are also known to support survival of AML cells and influence their response to chemotherapy agents.
Source: Clinical Lymphoma, Myeloma and Leukemia - Category: Hematology Authors: Source Type: research