Characterization of a long isoform of IL-1R8 (TIR8/SIGIRR).

Characterization of a long isoform of IL-1R8 (TIR8/SIGIRR).
Eur Cytokine Netw. 2017 Jun 01;28(2):63-69
Authors: Vilia MG, Tocchetti M, Fonte E, Sana I, Muzio M
Abstract
IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence between the TIR domain and the C-terminal portion of the protein. IL-1R8 Long (IL-1R8L1) mRNA was specifically expressed and regulated in distinct cell lines, in a manner similar to the classic isoform. Overexpression of IL-1R8L1 resulted in the production of a corresponding protein that showed a pattern of cell localization similar to the classic isoform. An antibody directed against an IL-1R8L1 specific peptide, detected this novel isoform in different cell lines and tissues where this protein may complement the anti-inflammatory functions of classic IL-1R8.
PMID: 28840837 [PubMed - in process]

Source: European Cytokine Network - August 27, 2017 Category: Molecular Biology Tags: Eur Cytokine Netw Source Type: research