Barcoding of human haematopoietic stem and progenitor cells by whole genome sequencing reveals clonal dynamics of haematopoiesis

The number of hematopoietic stem cells (HSCs) in humans and the dynamics of how these contribute to blood over time are critical to our understanding of normal hematopoiesis and its subversion in disease. While molecular barcoding or lineage tracing approaches have been applied to assess the output of single HSCs in animal models, similar approaches have not been possible in unperturbed human hematopoiesis. To address this, we investigated hematopoietic dynamics by using somatic mutations as barcodes.

Source: Experimental Hematology - August 22, 2017 Category: Hematology Authors: Henry Lee-Six, Nina Friesgaard-Oebro, Mairi Shepherd, Sebastian Grossmann, Kevin Dawson, Miriam Belmonte, Robert Osborne, Inigo Martincorena, Carlos Gonzalez Arias, Jacob Grinfeld, Elisa Laurenti, Brian Huntly, Michael Stratton, Tony Green, David Kent, Pe Source Type: research