Coordinated Regulation of the EII(Man) and fruRKI Operons of Streptococcus mutans by Global and Fructose-Specific Pathways.
Coordinated Regulation of the EII(Man) and fruRKI Operons of Streptococcus mutans by Global and Fructose-Specific Pathways.
Appl Environ Microbiol. 2017 Aug 18;:
Authors: Zeng L, Chakraborty B, Farivar T, Burne RA
Abstract
The glucose/mannose-PTS permease EII(Man) encoded by manLMN in the dental caries pathogen Streptococcus mutans has a dominant influence on sugar-specific, CcpA-independent catabolite repression (CR). Mutations in manL affect energy metabolism and virulence-associated traits, including biofilm formation, acid tolerance and competence. Using promoter:reporter fusions, expression of the manLMN and the fruRKI operons, encoding a transcriptional regulator, a fructose-1-P kinase and a fructose-PTS permease EII(Fru), respectively, was monitored in response to carbohydrate source and in mutants lacking CcpA, FruR, and components of EII(Man) Expression of genes for EII(Man) and EII(Fru) was directly regulated by CcpA and CR, as evinced by in vivo and in vitro methods. Unexpectedly, not only was the fruRKI operon negatively regulated by FruR, but so was manLMN Carbohydrate transport by EII(Man) had a negative influence on expression of manLMN, but not fruRKI In agreement with the proposed role of FruR in regulating these PTS operons, loss of fruR or fruK substantially altered growth on a number of carbohydrates, including fructose. RNA deep sequencing revealed profound changes in gene regulation caused by deletion of fruK or...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Zeng L, Chakraborty B, Farivar T, Burne RA Tags: Appl Environ Microbiol Source Type: research
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