In vitro toxicological assessment of Arrabidaea brachypoda (DC.) Bureau: Mutagenicity and estrogenicity studies

Publication date: Available online 18 August 2017 Source:Regulatory Toxicology and Pharmacology Author(s): Flávia Aparecida Resende, Catarine Haidê Nogueira, Lívia Greghi Espanha, Paula Karina Boldrin, Ana Paula Oliveira-Höhne, Mariana Santoro de Camargo, Cláudia Quintino da Rocha, Wagner Vilegas, Eliana Aparecida Varanda Arrabidaea brachypoda (DC.) Bureau is a shrub native Cerrado, known as "cipó-una", "tintureiro" or “cervejinha do campo” and popularly used in Southeastern and Northeastern Brazil to treatment of kidney stones and painful joints (arthritis). Nevertheless, scientific information regarding this species is scarce, and there are no reports related to its possible estrogenic and mutagenic effects. Thus, the principal objective of this study was to assess the mutagenic and estrogenic activities of the hydroalcoholic extracts of the leaves, stalks, roots, their respective fractions and isolated compounds of A. brachypoda. The mutagenic activity was evaluated by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, in the absence (-S9) and presence (+S9) of metabolic activation system. In the RYA was used Saccharomyces cerevisiae engineered strain BY4741 (MATaura3Δ0 leu2Δ0 his3Δ1 met15Δ0) which reproduce the natural pathway of genetic control by estrogens in vertebrate cells; it has the advantage of its simplicity and a high throughput. All extracts and aqueous fraction of leaves A. brachypoda were mutagenic. The crud...
Source: Regulatory Toxicology and Pharmacology - Category: Toxicology Source Type: research