Complete genome sequence of a novel, virulent Ahjdlikevirus bacteriophage that infects Enterococcus faecium

AbstractA novel virulent bacteriophage named vB_EfaP_IME199 that specifically infectsEnterococcus faecium was isolated and characterized. Its optimal multiplicity of infection was 0.01, and it had a 30  minute outbreak period. High-throughput sequencing revealed that the phage has a dsDNA genome of 18,838 bp with 22 open reading frames. The genome has very low homology to all other bacteriophage sequences in the GenBank database. Run-off sequencing experiments confirmed that vB_EfaP_IME199 has s hort inverted terminal repeats. Phylogenetic analysis indicated that vB_EfaP_IME199 can be taxonomically classified as a new member of the genusAhjdlikevirus of familyPodoviridae.
Source: Archives of Virology - Category: Virology Source Type: research