Modeling carbon dioxide effect in a controlled atmosphere and its interactions with temperature and pH on the growth of L.  monocytogenes and P. fluorescens.

Modeling carbon dioxide effect in a controlled atmosphere and its interactions with temperature and pH on the growth of L. monocytogenes and P. fluorescens. Food Microbiol. 2017 Dec;68:89-96 Authors: Couvert O, Guégan S, Hézard B, Huchet V, Lintz A, Thuault D, Stahl V Abstract The effect of carbon dioxide, temperature, and pH on growth of Listeria monocytogenes and Pseudomonas fluorescens was studied, following a protocol to monitor microbial growth under a constant gas composition. In this way, the CO2 dissolution didn't modify the partial pressures in the gas phase. Growth curves were acquired at different temperatures (8, 12, 22 and 37 °C), pH (5.5 and 7) and CO2 concentration in the gas phase (0, 20, 40, 60, 80, 100% of the atmospheric pressure, and over 1 bar). These three factors greatly influenced the growth rate of L. monocytogenes and P. fluorescens, and significant interactions have been observed between the carbon dioxide and the temperature effects. Results showed no significant effect of the CO2 concentration at 37 °C, which may be attributed to low CO2 solubility at high temperature. An inhibitory effect of CO2 appeared at lower temperatures (8 and 12 °C). Regardless of the temperature, the gaseous CO2 is sparingly soluble at acid pH. However, the CO2 inhibition was not significantly different between pH 5.5 and pH 7. Considering the pKa of the carbonic acid, these results showed the dissolved carbon under ...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research