Correlation analysis of surfactant protein A and surfactant protein D with lung function in exhaled breath condensate from lung cancer patients with and without COPD.

Correlation analysis of surfactant protein A and surfactant protein D with lung function in exhaled breath condensate from lung cancer patients with and without COPD. Mol Med Rep. 2017 Aug 07;: Authors: Lin X, Wu Z, Fan Y, Chi M, Wang X, Zhang X, Sun D Abstract Pulmonary surfactant protein A (SP‑A) and pulmonary surfactant protein D (SP‑D) are associated with the pathogenesis of chronic obstructive pulmonary disease (COPD). The aim of the present study was to determine the correlation between SP‑A, SP‑D and lung function in patients with COPD. A total of 60 patients with lung cancer undergoing unilateral lobectomy were selected and divided into three groups, including a non‑COPD group (n=20), a COPD treatment group (n=20) and a COPD control group (n=20). The levels of SP‑A and SP‑D were detected in the exhaled breath condensate (EBC) using ELISA analysis. Tissue samples were obtained during lobectomy via resection of the adjacent lung tissues, located >5 cm from the nodule. Immunohistochemistry and reverse transcription‑quantitative polymerase chain reaction analysis was performed. The proportion of SP‑A+ alveolar type II (ATII) cells and the mRNA levels of SP‑A and SP‑D in lung tissue were measured. In addition, the correlation between SP‑A and SP‑D in EBC, SP‑A and SP‑D mRNA in lung tissue, forced expiratory volume in 1 sec (FEV1) and the ratio of SP‑A+ ATII, was evaluated. The expression level...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research