Fluorescent CdSe QDs containing Bacillus licheniformis bioprobes for Copper (II) detection in water

In this study, we reported that CdSe QDs could be bio-synthesized in Bacillus licheniformis. After optimization, the obtained CdSe QDs exhibited a uniform particle size of 3.71±0.04nm with a maximum fluorescence emission wavelength at 550nm and the synthetical positive ratio can reach up to 87%. Spectral properties, constitution, particle sizes and crystalline phases of the CdSe QDs were systematically and integrally investigated. The CdSe QD-containing Bacillus licheniformis cells were further used as whole fluorescent bio-probes to detect copper (II) (Cu2+) in water, which demonstrated a low limit of detection (0.91μM). The assay also showed a good selectivity for Cu2+ over other ions including Al3+, Cd2+, Mg2+, K+, Na+, NH4 +, Zn2+, CH3COO+, Pb2+ and I−. Our study suggests the fluorescent CdSe QDs-containing Bacillus licheniformis bio-probes as a promising approach for detection of Cu2+ in complex solution environment.
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research