Evolving beta-lactamase epidemiology in Enterobacteriaceae from Italian nationwide surveillance, October 2013: KPC-carbapenemase spreading among outpatients

This study investigated the presence of these resistance mechanisms in consecutive non-replicate isolates ofEscherichia coli (n?=?2,352),Klebsiella pneumoniae (n?=?697), andProteus mirabilis (n?=?275) from an Italian nationwide cross-sectional survey carried out in October 2013. Overall, 15.3% of isolates were non-susceptible to extended-spectrum cephalosporins but susceptible to carbapenems (ESCR-carbaS), while 4.3% were also non-susceptible to carbapenems (ESCR-carbaR). ESCR-carbaS isolates were contributed by all three species, with higher proportions among isolates from inpatients (20.3%) but remarkable proportions also among those from outpatients (11.1%). Most ESCR-carbaS isolates were ESBL-positive (90.5%), and most of them were contributed byE. coli carryingblaCTX-M group 1 genes. Acquired ACBLs were less common and mostly detected inP. mirabilis. ESCR-carbaR isolates were mostly contributed byK. pneumoniae (25.1% and 7.7% amongK. pneumoniae isolates from inpatients and outpatients, respectively), withblaKPC as the most common carbapenemase gene. Results showed an increasing trend for both ESBL and carbapenemase producers in comparison with previous Italian surveys, also among outpatients.
Source: Eurosurveillance - Category: Infectious Diseases Source Type: research

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CONCLUSION: We believe that the detection of microorganisms presenting genetic elements in environments such as water is necessary for the prevention and control of their dissemination with potential to infect humans and other animals in eventual contact with these environments. PMID: 31529856 [PubMed - in process]
Source: Biomedica : Revista del Instituto Nacional de Salud - Category: International Medicine & Public Health Tags: Biomedica Source Type: research
CONCLUSIONS: The high frequency of multidrug-resistant ESBL-producing isolates should alert the regional health authorities to take measures to reduce the risk of outbreaks caused by these types of bacteria in the community. PMID: 31529852 [PubMed - in process]
Source: Biomedica : Revista del Instituto Nacional de Salud - Category: International Medicine & Public Health Tags: Biomedica Source Type: research
A common Salmonella Enteritidis sequence type from poultry and human gastroenteritis in Ibagué, Colombia Biomedica. 2019 05 01;39(s1):50-62 Authors: Fandiño LC, Verjan N Abstract Salmonella Enteritidis es una de las mayores causas de salmonelosis en el mundo, siendo los huevos contaminados y la carne de pollo cruda sus principales fuentes de infección. En Ibagué, Colombia, se identificaron los principales serovares circulando en granjas, superficies de huevos y canales de pollo, sin embargo, se desconoce si esos serovares son responsables de gastroenteritis. Objetivo. Evalu...
Source: Biomedica : Revista del Instituto Nacional de Salud - Category: International Medicine & Public Health Tags: Biomedica Source Type: research
Publication date: March 2020Source: Food Control, Volume 109Author(s): Quan Wang, MengYao Long, CaiYun Lv, SiPei Xin, XianGan Han, Wei JiangAbstractA simple, rapid, highly sensitive, and quantitative fluorescence-based immunochromatographic test (ICT) was successfully developed for the determination of Escherichia coli O157:H7 in food samples. Europium (III) chelated nanoparticles (EuNPs) conjugated to monoclonal antibodies specific to the O-specific polysaccharide fractions of E. coli O157:H7 were used as the fluorescence reporter. The fluorescence was visually observable within 15 min under dark conditions with an ultr...
Source: Food Control - Category: Food Science Source Type: research
Publication date: Available online 18 September 2019Source: Food BioscienceAuthor(s): Sarengaowa, Wenzhong Hu, Ke Feng, Zhilong Xiu, Aili Jiang, Ying LaoAbstractFresh-cut cantaloupe (Cucumis melo L.) is a popular food. However, the presence of foodborne pathogen on the cut surfaces leads to both spoilage and food poisoning of fresh-cut cantaloupe. The antibacterial effectiveness of an alginate-based edible coating (EC) containing thyme oil was evaluated against foodborne pathogens on fresh-cut cantaloupe samples. Cantaloupe samples were treated with alginate-based EC and alginate-based EC containing different concentration...
Source: Food Bioscience - Category: Food Science Source Type: research
Bhaskar Raju, Raunak P Parikh, Volker V Vetter, Shafi KolhapureIndian Journal of Public Health 2019 63(3):243-250 Rotavirus is a leading cause of severe pediatric diarrhea worldwide, with about 199,000 childhood deaths in 2015, of which 90% in low-income countries. India alone accounts for 22% of the global rotavirus gastroenteritis (RVGE)-related deaths among children below 5 years of age. The World Health Organization recommends introducing rotavirus vaccines (RVVs) as a priority in developing countries where high rates of RVGE are observed. To have the desired impact, RVV should be administered the earliest pos...
Source: Indian Journal of Public Health - Category: International Medicine & Public Health Authors: Source Type: research
In this study, a novel cold-adapted nitroreductase gene (psntr) was cloned from Antarctic sea-ice bacteria Psychrobacter sp. ANT206. The psntr gene was 813 bp in length and encoded a protein with flavin mononucleotide (FMN) binding sites. Homology modeling was performed to obtain structural information such as the longer loops and reduced amount of hydrogen bonds, which might be related to the high catalytic efficiency of PsNTR at low temperature. The psntr gene was successfully cloned in cold-shock pCold I vector and transformed to the expression host Escherichia coli (E. coli) BL21 with the induction by isopropyl β-...
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research
ConclusionThis study reported the prevalence and traits of clinical E. coli ST131 main subclones in Saudi Arabia. It also demonstrated the high prevalence of E. coli ST131 locally, and found different virulence genotypes and antimicrobial resistance phenotypes among ST131 subclones. In the future, preforming whole genome sequence-based studies on ST131 and its subclones is crucial to elucidate factors that drive the success of these organisms.
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research
Publication date: Available online 18 September 2019Source: Fish &Shellfish ImmunologyAuthor(s): Ruixia Wang, Xiucai Hu, Aijun Lü, Rongrong Liu, Jingfeng Sun, Yeong Yik Sung, Yajiao SongAbstractSkin plays an important role in the innate immune responses of fish, particularly towards bacterial infection. To understand the molecular mechanism of mucosal immunity of fish during bacterial challenge, a de novo transcriptome assembly of crucian carp Carassius auratus skin upon Aeromonas hydrophila infection was performed, the latter with Illumina Hiseq 2000 platform. A total of 118111 unigenes were generated and of thes...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Publication date: Available online 18 September 2019Source: Protein Expression and PurificationAuthor(s): Nazimuddin Khan, Jino Park, William L. Dean, Robert D. Gray, William Tse, Donghan Lee, T. Michael SaboAbstractThe protein ALL1 fused from chromosome 1q (AF1q) is overexpressed in a variety of cancers and acts to activate several signaling pathways that lead to oncogenesis. For example, AF1q has been shown to interact with T-cell Factor 7 (TCF7; also known as TCF1) from the Wnt/β-catenin pathway resulting in the transcriptional activation of the CD44 and the enhancement of breast cancer metastasis. Despite the impo...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
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