Identification of a Pseudomonas putida as biocontrol agent for tomato bacterial wilt disease

Publication date: Available online 27 July 2017 Source:Biological Control Author(s): Dongling Sun, Tao Zhuo, Xun Hu, Xiaojing Fan, Huasong Zou A bacterial isolate, A1, was collected from the rhizosphere soil of cultivated peanuts. Based on its 16S rRNA sequence, this isolate was identified as a Pseudomonas putida strain. On minimal medium supplemented with diverse nutrient substrates, the P. putida A1 strain could use fructose and fructosan, trehalose, and inositol as sole carbon resources. The ability of these four carbon resources, as well as leaf and root exudates, to stimulate cell migration in a chemotaxis assay was investigated. P. putida A1 was labelled with GFP to study colonization on the root surface; this strain was found to aggregate around wound sites. In addition to forming biofilms in vitro, A1 showed antimicrobial activity against several plant pathogenic bacteria, including Ralstonia solanacearum, Xanthomonas oryzae pv. oryzae, X. o. pv. oryzicola, and X. citri subsp. citri. In evaluations of biocontrol potential of tomato bacterial wilt, this isolate delayed the appearance of wilt symptoms for 4 days and reduced wilt disease severity. Overall, our results indicate that P. putida A1 could be an effective biocontrol agent for plant soil-borne diseases. Graphical abstract
Source: Biological Control - Category: Biology Source Type: research