Filament-like DREP4 CIDE domain: characterization and preliminary X-ray crystallographic studies

In this study, it was found that DREP4 CIDE domains form filament-like structures in solution. The length of the highly ordered filament-like structure is dependent on the salt concentration. By adjusting the salt concentration the DREP4 CIDE domain could be crystallized, and X-ray diffraction data were collected to a resolution of 1.9   Å . The crystals were found to belong to the orthorhombic space group P212121, with unit-cell parameters a = 53.08, b = 76.58, c = 174.59   Å .
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: apoptosis DNA fragmentation factor CIDE domain DREP4 research communications Source Type: research