In Vivo Enrichment of Diabetogenic T Cells

We describe a method to enrich and harvest autoimmune T cells in vivo by using a biomaterial scaffold loaded with protein antigens. In model antigen systems, we found that antigen-specific T cells become enriched within scaffolds containing their cognate antigens. When scaffolds containing lysates from an insulin-producing β-cell line were implanted subcutaneously in autoimmune diabetes–prone NOD mice, β-cell–reactive T cells homed to these scaffolds and became enriched. These T cells induced diabetes after adoptive transfer, indicating their pathogenicity. Furthermore, T-cell receptor (TCR) sequencing identified many expanded TCRs within the β-cell scaffolds that were also expanded within the pancreata of NOD mice. These data demonstrate the utility of biomaterial scaffolds loaded with disease-specific antigens to identify and study rare, therapeutically important T cells.

Source: Diabetes - July 21, 2017 Category: Endocrinology Authors: Thelin, M. A.; Kissler, S.; Vigneault, F.; Watters, A. L.; White, D.; Koshy, S. T.; Vermillion, S. A.; Mooney, D. J.; Serwold, T.; Ali, O. A. Tags: Immunology Immunology and Transplantation Source Type: research