Response to Comments on "The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport"

Baranovskiy et al. and Pellegrini argue that, based on structural data, the path for charge transfer through the [4Fe4S] domain of primase is not feasible. Our manuscript presents electrochemical data directly showing charge transport through DNA to the [4Fe4S] cluster of a primase p58C construct and a reversible switch in the DNA-bound signal with oxidation/reduction, which is inhibited by mutation of three tyrosine residues. Although the dispositions of tyrosines differ in different constructs, all are within range for microsecond electron transfer.

Source: ScienceNOW - July 20, 2017 Category: Science Authors: OBrien, E., Holt, M. E., Thompson, M. K., Salay, L. E., Ehlinger, A. C., Chazin, W. J., Barton, J. K. Tags: Biochemistry, Online Only t-comment Source Type: news