Regulatory effects of the novel synthesized Indole-3-carbaldehyde on expression of cell cycle genes: A study on Cyclin D and P21 expression by acute promylocytic leukemia cell line (NB4).

Regulatory effects of the novel synthesized Indole-3-carbaldehyde on expression of cell cycle genes: A study on Cyclin D and P21 expression by acute promylocytic leukemia cell line (NB4). Cell Mol Biol (Noisy-le-grand). 2017 May 20;63(5):60-67 Authors: Karimabad MN, Mahmoodi M, Jafarzadeh A, Darekordi A, Hajizadeh MR, Khorramdelazad H, Sayadi AR, Khanamani Falahati-Pour S, Hassanshahi G Abstract A large variety of heterocycles are known to date and among these, indole and pyran rings are of particular interest. The present investigation was aimed to investigate the effects of novel Indole-3-carbaldehyde derivative (NI-3-CD) analogue on growth inhibition of cells and its regulatory effects on expression of cell cycle genes in acute promylocytic leukemia (APL) cell line. NB4 cells were cultured in presence of RPMI1640 medium contained various concentrations of NI-3-CD and basal compound of indole (I3C) (15.12-1000 µg/mL) for 24, 48 and 72 hours. The inhibitory effects of compound on cellular proliferation were assessed by both trypan blue staining and MTT assay techniques. when was confirmed apoptosis the changes in expression of Cyclin D and P21 were determined by quantitative Real-Time PCR. Western blotting analysis was also applied for evaluating the expression of P21 at protein level. Differences were considered significant if p values less than 0.05. Our results showed a significant difference between various concentrations of NI...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research